An interlaboratory comparison of transformation in Syrian hamster embryo cells with model and coded chemicals.
An interlaboratory comparison of transformation in Syrian hamster embryo cells with model and coded chemicals.
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叙利亚仓鼠胚胎细胞转化与模型和编码化学物质的实验室间比较。
DOI：
10.1002/em.2860080108
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Huberman,E
中科院分区：
文献类型：
--
作者：
Tu,A;Hallowell,W;Pallotta,S;Sivak,A;Lubet,RA;Curren,RD;Avery,MD;Jones,C;Sedita,BA;Huberman,E
三个独立实验室在叙利亚仓鼠胚胎克隆转化试验系统中检测了8种“模型”和5种编码化学品，以确定系统的实验室内和实验室间重现性，并确定变异性来源。当使用共同的细胞池和相同批次的胎牛血清时，三个实验室对八种模型化学物质的活性达成了共识：五种化学物质（苯并（a）芘、7，12-二甲基苯并（a）蒽、N-甲基-N′-硝基-N-亚硝基胍、硝基喹啉-N-氧化物和铬酸铅）诱导形态转化，而无外源性代谢活化，三种（N-2-芴基乙酰胺、芘和蒽）不产生转化反应。五种编码化学品（2，6-二氯对苯二胺、4，4 ′-二氨基二苯醚、邻氨基苯甲酸肉桂酯、敌敌畏和利血平）代表环境化学品类别，但不一定是强致癌物，在本实验室间研究中产生了更不明确的反应。因此，虽然在某些情况下，该试验可用于区分转化和非转化化学品，但低转化频率和获得任何剂量反应结果的固有限制是目前在常规检测项目中使用该系统的主要限制。增加转化频率或扩大转化表型的表达的努力构成了为了克服这些限制而应该探索的一些方法。
Three independent laboratories tested eight “model” and five coded chemicals in the Syrian hamster embryo clonal transformation assay system to establish the intra‐ and interlaboratory reproducibility of the system and to identify sources of variability. When a common cell pool and the same lot of fetal calf serum were used, the three laboratories obtained consensus on the activity of eight model chemicals: five chemicals (benzo(a)pyrene, 7,12‐dimethylbenz(a)anthracene, N‐methyl‐N′‐nitro‐N‐nitrosoguanidine, nitroquinoline‐N‐oxide, and lead chromate) induced morphological transformation without exogenous metabolic activation and three (N‐2‐fluorenylacetamide, pyrene, and anthracene) produced no transformation response. Five coded chemicals (2,6‐dichloro p‐phenylenediamine, 4,4′‐oxydianiline, cinnamyl anthranilate, dichlorvos, and reserpine), representative of environmental chemical classes, but not necessarily strong carcinogens, produced more equivocal responses in this interlaboratory study. Thus, while the assay can be used to distinguish between transforming and nontransforming chemicals in some cases, the intrinsic limitations in low transformation frequency and in achieving any dose‐response results are major constraints to the use of this systsem in a routine testing program at the present time. Efforts to increase the transformation frequency or to amplify the expression of the transformed phenotype constitute some of the approaches which should be explored in order to overcome these limitations.