Additive Manufacturing of Catalytically Active Living Materials
Additive Manufacturing of Catalytically Active Living Materials
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DOI：
10.1021/acsami.8b02719
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发表时间：
2018-04-25
影响因子：
9.5
通讯作者：
Nelson, Alshakim
中科院分区：
文献类型：
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作者：
Saha, Abhijit;Johnston, Trevor G.;Nelson, Alshakim
活性材料是由居住在聚合物基质中的活细胞组成的复合材料，旨在利用细胞的固有功能来解决广泛的应用，如发酵和生物传感。在这里，我们展示了用于连续发酵的催化活性生物材料（AMCALM）的增材制造。开发了一种基于f127 -二甲丙烯酸酯的多刺激响应酵母负载水凝胶墨水，并使用直写3D打印机进行了打印。聚合物水凝胶墨水对温度和压力的可逆刺激响应行为是至关重要的，因为它们使酵母细胞的容易结合和随后的3D晶格结构的制造成为可能。随后印刷聚合物水凝胶的光交联提供了一种坚固的弹性材料。这些酵母负载的活材料在连续批处理中将葡萄糖发酵成乙醇的过程中代谢活跃2周，效率没有显著降低（类似于90%的乙醇收率）。该细胞固定化平台可能适用于其他转基因酵母菌株在连续生物发酵过程中生产其他高价值化学品。
Living materials, which are composites of living cells residing in a polymeric matrix, are designed to utilize the innate functionalities of the cells to address a broad range of applications such as fermentation and biosensing. Herein, we demonstrate the additive manufacturing of catalytically active living materials (AMCALM) for continuous fermentation. A multi-stimuli-responsive yeast-laden hydrogel ink, based on F127-dimethacrylate, was developed and printed using a direct-write 3D printer. The reversible stimuli-responsive behaviors of the polymer hydrogel inks to temperature and pressure are critical, as they enabled the facile incorporation of yeast cells and subsequent fabrication of 3D lattice constructs. Subsequent photo-cross-linking of the printed polymer hydrogel afforded a robust elastic material. These yeast-laden living materials were metabolically active in the fermentation of glucose into ethanol for 2 weeks in a continuous batch process without significant reduction in efficiency (similar to 90% yield of ethanol). This cell immobilization platform may potentially be applicable toward other genetically modified yeast strains to produce other high-value chemicals in a continuous biofermentation process.