Repeated administration of methamphetamine blocked cholecystokinin-octapeptide injection-induced c-fos mRNA expression without change in capsaicin-induced junD mRNA expression in rat cerebellum
Repeated administration of methamphetamine blocked cholecystokinin-octapeptide injection-induced c-fos mRNA expression without change in capsaicin-induced junD mRNA expression in rat cerebellum
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DOI：
10.1007/s00702-010-0444-1
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发表时间：
2010-09-01
影响因子：
3.3
通讯作者：
Fukumaki, Yasuyuki
中科院分区：
文献类型：
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作者：
Hamamura, Mitsuko;Ozawa, Hidetoshi;Fukumaki, Yasuyuki
在小脑中，有许多含胆囊收缩素（CCK-8）的纤维。由于全身性CCK-8注射诱导的焦虑（心理应激）激活蓝斑细胞，将苔藓纤维输入到小脑，我们研究了全身性CCK-8注射是否激活大鼠和小鼠小脑。首先，注射CCK-8被发现诱导c-fos mRNA的表达在一个模糊的斑片状的模式，这是不同于单一甲基苯丙胺诱导的Zeolyband-like c-fos mRNA的表达，这表明CCK-8激活苔藓纤维诱导基因表达不同的多巴胺含苔藓纤维在腹侧被盖区。第二，由于CCK-8促进中脑多巴胺的神经活动，我们研究了是否重复甲基苯丙胺给药，诱导行为敏化小脑CCK系统有类似的效果。重复给予甲基苯丙胺抑制CCK-8诱导的大鼠小脑c-fos mRNA表达。第三，辣椒素注射（物理应激）到大鼠后肢增加junD mRNA的表达，c-fos mRNA的表达没有影响，重复甲基苯丙胺注射辣椒素诱导的junD mRNA的表达没有影响。第四，无论是单独注射甲基苯丙胺还是CCK-8，小鼠蓝斑c-fos mRNA的表达均增加，因此去甲肾上腺素可能与CCK-8激活系统相互作用，而多巴胺则不起作用。然而，我们认为这种可能性不大。因此，我们得出结论，重复甲基苯丙胺管理，虽然多巴胺选择性抑制CCK-8注射后，在小脑c-fos mRNA的表达。
In the cerebellum, there are numerous cholecystokinin (CCK-8)-containing fibers. Since systemic CCK-8 injection-induced anxiety (psychological stress) activates the locus coeruleus cells that send mossy fiber inputs to the cerebellum, we examined whether systemic CCK-8 injections activate the rat and mouse cerebellum. First, injections of CCK-8 were found to induce c-fos mRNA expression in a vague patchy pattern that is different from single methamphetamine-induced Zebrin band-like c-fos mRNA expression, suggesting that the CCK-8 activating mossy fibers induce gene expression differently from the dopamine-containing mossy fibers in the ventral tegmental area. Second, since CCK-8 facilitates neural activity of dopamine in the midbrain, we examined whether repeated methamphetamine administration that induced behavioral sensitization had similar effects on the cerebellar CCK system. Repeated administration of methamphetamine suppressed the CCK-8-induced c-fos mRNA expression in the rat cerebellum. Third, capsaicin injections (physical stress) into a hind limb of the rat increased junD mRNA expression with no effect on c-fos mRNA expression, and repeated methamphetamine injections had no effect on the capsaicin-induced expression of junD mRNA. Fourth, either single injection of methamphetamine or CCK-8 to mice increased c-fos mRNA expression in the locus coeruleus, and so noradrenalin, but not dopamine, might interact with CCK-8-activating system. However, we considered the possibility unlikely. Thus, we conclude that repeated methamphetamine administration though dopamine selectively inhibits the c-fos mRNA expression after CCK-8 injection in the cerebellum.