VIRALLY CODED NONCAPSID PROTEIN ASSOCIATED WITH BOVINE PARVOVIRUS INFECTION
VIRALLY CODED NONCAPSID PROTEIN ASSOCIATED WITH BOVINE PARVOVIRUS INFECTION
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DOI：
10.1128/jvi.49.2.315-318.1984
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发表时间：
1984-01-01
影响因子：
5.4
通讯作者：
BATES, RC
中科院分区：
文献类型：
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作者：
LEDERMAN, M;PATTON, JT;BATES, RC
在牛细小病毒（BPV）感染细胞的细胞核中检测到与染色质相关的磷酸化蛋白（NP-1），分子量为28,000。尽管bpv特异性蛋白与兔细小病毒或腺相关病毒感染相关的非衣壳蛋白在大小上相似，但在适当的细胞感染几种自主啮齿动物细小病毒后，未检测到该大小范围内的蛋白。通过对胰凝乳酶蛋白水解产物的电泳分析，可以发现NP-1与BPV衣壳蛋白在结构上的同源性。该蛋白可以在体外翻译感染bpv的胎牛肺细胞的RNA和bpv特异性RNA后检测到。在体内和体外合成的物种之间的同源性是通过相似的胰凝乳产物来证明的。
A phosphorylated protein (NP-1) with a MW of 28,000 has been detected in nuclei of bovine parvovirus (BPV)-infected cells in association with chromatin. No protein in this size range was detected after infection of appropriate cells with several autonomous rodent parvoviruses although the BPV-specific protein is similar in size to noncapsid proteins associated with rabbit parvovirus or adeno-associated virus infection. Structural homology between NP-1 and a BPV capsid protein could be detected by electrophoretic analysis of the products of proteolysis with chymotrypsin. This protein can be detected after in vitro translation of RNA from BPV-infected fetal bovine lung cells and BPV-specific RNA. Homology between the in vivo- and in vitro-synthesized species was shown by the similarity of the chymotryptic products.