Characterization of Helicobacter pylori tlyA and Its Association with Bacterial Density
Characterization of Helicobacter pylori tlyA and Its Association with Bacterial Density
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幽门螺杆菌的特征及其与细菌密度的关系
DOI：
10.1159/000518538
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发表时间：
2021
影响因子：
2.3
通讯作者：
Yamaoka Yoshio
中科院分区：
文献类型：
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作者：
Prakosa Adi Wasis;Miftahussurur Muhammad;Juniastuti Juniastuti;Waskito Langgeng Agung;Doohan Dalla;Fauzia Kartika Afrida;Rezkitha Yudith Annisa Ayu;Sugihartono Titong;Syam Ari Fahrial;Uchida Tomohisa;Yamaoka Yoshio
背景在最近的研究中，一种毒力较低的幽门螺杆菌变异体仍然可以在人的胃中定植并诱导胃炎症，提示其他毒力因子参与其中，如TlyA溶血素。然而，TlyA在幽门螺杆菌感染发病机制中的关联仍不清楚。我们调查了tlyA谱，并确定了它与胃炎严重程度的关系。方法使用DNA库和以前研究的二次数据进行观察性研究。用NGS检测tlyA变异，并用聚合酶链式反应进行确认。胃炎的严重程度按最新的悉尼系统分类。用χ2检验或FISHER精确检验对离散变量进行检验。结果发现2株Hp tlyA变异株，分别为tlyA1和tlyA2。我们注意到在氨基酸序列32-35中有一个独特的变异，该变异仅在从巴布亚岛分离的幽门螺杆菌中检测到。此外，我们还观察到tlyA变异与胃窦内幽门螺杆菌的密度显著相关(p=0.002)。组织学分析显示，TlyA1与Hp密度高于TlyA2。然而，我们没有观察到tlyA与炎症细胞的渗透有任何显著的联系。结论我们观察到两个tlyA变异(tlyA1和tlyA2)。TlyA与细菌密度的显著相关性表明，tlyA在胃粘膜定植过程中的作用比它对胃粘膜炎症程度的影响更重要。
BackgroundIn the recent studies, a less virulent Helicobacter pylori variant could still colonize the human stomach and induce gastric inflammation, suggesting the involvement of other virulence factors, such as TlyA hemolysin. Nevertheless, the association of TlyA in the pathogenesis of H. pylori infection remains unclear. We investigated the tlyA profile and determined its relationship with gastritis severity.MethodsAn observational study was conducted using DNA stocks and secondary data from previous studies. The tlyA variant was examined by NGS and confirmed with polymerase chain reaction. Gastritis severity was categorized by the Updated Sydney System. The relationship between a variant of tlyA and gastritis severity was determined, in which discrete variables were tested using the χ 2 test or Fisher exact test.ResultsTwo H. pylori tlyA variants were observed and characterized as tlyA1 and tlyA2. We noted a unique variant in the amino acid sequence 32–35 that is exclusively detected among H. pylori isolated from the Papua island. In addition, we observed that the tlyA variant had a significant association with the H. pylori density in the antral (p= 0.002). Histological analyses revealed that TlyA1 was associated with higher H. pylori density than TlyA2. However, we did not observe any significant association of tlyA with the infiltration of inflammation cells.ConclusionsWe observed 2 tlyA variants (tlyA1 and tlyA2). A significant association of tlyA with bacterial density suggested that tlyA plays a more significant role in the colonization process than its influence on the severity of inflammation in gastric mucosa.