Interaction of ACE2 and integrin β1 in failing human heart
Interaction of ACE2 and integrin β1 in failing human heart
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DOI：
10.1016/j.bbadis.2004.05.005
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发表时间：
2004-08-04
影响因子：
6.2
通讯作者：
Zisman, LS
中科院分区：
文献类型：
--
作者：
Lin, QS;Keller, RS;Zisman, LS
通过免疫沉淀、Western blotting、活性测定和ESI串联质谱分析，发现从衰竭的人心脏中纯化的ACE2与整合素β 1形成复合物。ACE2/整合素复合物纯化酶的K-m为6.8 muM， V-max为2.13 pmol/min/mul。以Ang II为底物，pH为7.5时活性最佳。(C) 2004 Elsevier B.V.版权所有
ACE2 purified from failing human heart was found to form a complex with integrin beta1 by immunoprecipitation, Western blotting, activity assay, and ESI tandem mass spectroscopy. The ACE2/integrin complex showed a K-m of 6.8 muM and a V-max of 2.13 pmol/min/mul purified enzyme. Activity was optimal at pH 7.5 with Ang II substrate. (C) 2004 Elsevier B.V. All rights reserved.