Nano-model membrane filters for the well-controlled separation of biomolecules
Nano-model membrane filters for the well-controlled separation of biomolecules
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DOI：
10.1016/j.colsurfa.2010.12.015
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发表时间：
2011-03
期刊：
影响因子：
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通讯作者：
S. El‐Safty;A. Shahat;H. Nguyen
中科院分区：
文献类型：
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作者：
S. El‐Safty;A. Shahat;H. Nguyen
将蛋白质分离成相对均匀的组和大小在生物制药中非常重要。我们开发了一种简单而通用的方法，使用阳极氧化铝膜（AAM）作为人质袋，在二氧化硅纳米管（NT）内设计立方介孔笼。三维介孔结构具有 17.3nm 的大晶胞常数和沿垂直于二氧化硅 NT 轴的方向均匀的孔入口（∼5nm），允许开发尺寸专有的纳米过滤膜作为分离蛋白质（如溶菌酶、肌红蛋白、β-乳球蛋白和血红蛋白）的强大工具。笼状二氧化硅 NT 在 AAM 口袋人质的纳米孔内合成。三嵌段共聚物 F127 (PEO100PPO70PEO100) 的四元微乳液液晶相用于模板引导合成 NT 内的立方 Im3m 介孔。因此，开发由垂直于纳米通道纵轴的3D介孔二氧化硅NT组成的纳滤膜将有效增强基于尺寸的蛋白质分离。在这种纳米过滤器设计中，在添加表面活性剂F127/二氧化硅组合物域之前，用极性硅烷偶联剂（例如N-三甲氧基甲硅烷基丙基-N,N,N-三甲基氯化铵）涂覆AAM的孔道，有利于生产极其坚固的膜结构序列，而不会在二氧化硅NT和AAM壁之间形成气隙。我们的纳米过滤器能够用有机部分（例如三甲基氯硅烷）对形成的二氧化硅NT的纳米级孔表面进行精确修饰，从而有效过滤高浓度的蛋白质（滞留物≥10−4moldm−3），而没有明显的动力学阻碍。
The separation of proteins into relatively homogeneous groups and sizes is important in biopharmaceuticals. We developed a simple yet general method for engineering cubic mesopore cages inside silica nanotubes (NTs) using an anodic alumina membrane (AAM) as hostage pockets. The three-dimensional mesocage structures with a large unit cell constant of 17.3nm and uniform pore entrance (∼5nm) running in the direction perpendicular to the axis of the silica NTs allow the development of size-exclusive nanofilter membranes as a powerful tool for the separation of proteins, such as lysozyme, myoglobin, β-lactoglobulin, and hemoglobin. Cage silica NTs were synthesized within the nanopore of AAM pocket hostages. Quaternary microemulsion liquid crystalline phases of triblock copolymer F127 (PEO100PPO70PEO100) were used in the template-guided synthesis of cubic Im3m mesocage pores inside the NTs. Thus, the development of nanofilter membranes composed of 3D mesocage silica NTs perpendicular to the longitudinal axis of the nanochannels would effectively enhance the size-based separation of proteins. In such nanofilter design, the coating of the pore channels of the AAM with polar silane coupling agents, such as N-trimethoxysilylpropyl-N,N,N-trimethylammonium chloride, prior to the addition of surfactant F127/silica composition domains facilitate the production of extremely robust constructed sequences of membranes without the formation of air gaps between silica NTs and AAM walls. Our nanofilter enables the precise modification of the nanoscale pore surfaces of the formed silica NTs with organic moieties, such as trimethylchlorosilane, leading to the efficient filtration of high concentrations of proteins (retentate≥10−4moldm−3) without substantial kinetic hindrance.