Species-level identification of isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex by sequence analysis of the 16S-23S rRNA gene spacer region
Species-level identification of isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex by sequence analysis of the 16S-23S rRNA gene spacer region
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DOI：
10.1128/jcm.43.4.1632-1639.2005
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发表时间：
2005-04-01
影响因子：
9.4
通讯作者：
Chang, TC
中科院分区：
文献类型：
--
作者：
Chang, HC;Wei, YF;Chang, TC
醋酸钙不动杆菌A.包括在醋酸钙不动杆菌-鲍曼不动杆菌复合体中的鲍曼不动杆菌、基因组物种3和基因组物种13 TU在遗传上高度相关，并且难以在表型上区分。除了A。醋酸钙球菌，它们都是重要的医院菌种。本研究探讨了16 S-23 SrRNA基因间隔区（ITS）序列在区分中国对虾（A.醋酸钙A.评价鲍曼不动杆菌复合物。对该复合菌的11株参考菌株和17株其他不动杆菌属的ITS序列进行了测定。ITS长度（607 ~ 638 bp）和序列在A.醋酸钙A.鲍曼氏复合体种内ITS序列相似性范围为0.99至1.0，而种间相似性范围为0.86至0.92。根据这些标准，79株临床分离物被鉴定为A.醋酸钙杆菌（18株）或A.用API 20 NE系统（bioMerieux Vitek，Marcy I 'Etoile，France）鉴定鲍曼不动杆菌（61株）为A.鲍曼不动杆菌（46个分离株）、基因组种3（19个分离株）和基因组种13 TU（11个分离株）。利用ITS序列分析对79个菌株进行鉴定，鉴定率为96.2%（76/79）。醋酸钙A.鲍曼不动杆菌复合物，并通过扩增的rRNA基因限制性分析和基因组DNA分析，通过使用参考菌株的谱库进行AFLP分析，证实了该方法的准确性。总之，基于ITS序列的鉴定是可靠的，并为阐明不同种的A.醋酸钙A.鲍曼氏复合体
The species Acinetobacter calcoaceticus, A. baumannii, genomic species 3, and genomic species 13TU included in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex are genetically highly related and difficult to distinguish phenotypically. Except for A. calcoaceticus, they are all important nosocomial species. In the present study, the usefulness of the 16S-23S rRNA gene intergenic spacer (ITS) sequence for the differentiation of (genomic) species in the A. calcoaceticus-A. baumannii complex was evaluated. The ITSs of 11 reference strains of the complex and 17 strains of other (genomic) species of Acinetobacter were sequenced. The ITS lengths (607 to 638 bp) and sequences were highly conserved for strains within the A. calcoaceticus-A. baumannii complex. Intraspecies ITS sequence similarities ranged from 0.99 to 1.0, whereas interspecies similarities varied from 0.86 to 0.92. By using these criteria, 79 clinical isolates identified as A. calcoaceticus (18 isolates) or A. baumannii (61 isolates) with the API 20 NE system (bioMerieux Vitek, Marcy I'Etoile, France) were identified as A. baumannii (46 isolates), genomic species 3 (19 isolates), and genomic species 13TU (11 isolates) by ITS sequencing. An identification rate of 96.2% (76 of 79 isolates) was obtained by using ITS sequence analysis for identification of isolates in the A. calcoaceticus-A. baumannii complex, and the accuracy of the method was confirmed for a subset of strains by amplified rRNA gene restriction analysis and genomic DNA analysis by AFLP analysis by using libraries of profiles of reference strains. In conclusion, ITS sequence-based identification is reliable and provides a promising tool for elucidation of the clinical significance of the different species of the A. calcoaceticus-A. baumannii complex.