Colour-coded pellets increase the sensitivity of the staircase test to differentiate skilled forelimb performances of control and 6-hydroxydopamine lesioned rats
Colour-coded pellets increase the sensitivity of the staircase test to differentiate skilled forelimb performances of control and 6-hydroxydopamine lesioned rats
复制标题
DOI：
10.1016/j.brainresbull.2006.04.006
复制
发表时间：
2006-06-15
影响因子：
3.8
通讯作者：
Nikkliah, Guido
中科院分区：
文献类型：
--
作者：
Kloth, Verena;Klein, Alexander;Nikkliah, Guido
蒙托亚楼梯测试此前曾被用来研究小鼠和大鼠在中枢神经系统不同部位发生病变和细胞植入后的熟练前肢表现。在这里，我们描述了对原始测试设计的修改，其中为每个步骤引入了不同颜色的食物颗粒，并展示了新方法和修改方法的结果。在本研究中，使用单侧 6-羟基多巴胺 (6-OHDA) 损伤大鼠和健康对照大鼠。对伸手和抓握动作的新评估考虑了伸手难度的不同程度。彩色食物颗粒代表楼梯的不同台阶。原始测试方法与修改后的测试方法之间的比较揭示了较低步骤上最显着的显着差异。值得注意的是，偏侧帕金森病大鼠的抓握运动模式从精确地触及（病变前）变为拖着脚步，但未能成功地尝试触及颗粒。通过对原始楼梯测试的评估无法获得对这种行为变化的观察。总之，改进的楼梯测试引入了颜色编码的颗粒系统，该系统明显提高了测试灵敏度，并揭示了对帕金森病大鼠模型中熟练前肢使用的新见解。因此，它可能成为未来研究中与可塑性引起的熟练前肢伸展和抓握运动变化相关的有价值的工具。 (c) 2006 Elsevier Inc. 保留所有权利。
The Montoya staircase test has previously been used to study the skilled forelimb performance of mice and rats following lesions and cell implants in different parts of the central nervous system. Here we describe a modification of the original test design which introduces differently coloured food pellets for each step, and present the results of the new and modified method. In this study unilaterally 6-hydroxydopamine (6-OHDA) lesioned rats and healthy control rats were used. The new evaluation of reaching and grasping movements takes into consideration the various levels of reaching difficulty. The coloured food pellets code for different steps of the staircase. The comparison between the original versus the modified test methods revealed significant differences most prominently on the lower steps. It is important to notice that the pattern of grasping movements in the hemiparkinsonian rats changes from precise reaching (prior to lesion) to shuffling and unsuccessfully trying to reach pellets. The observation of this change in behaviour would not have been obtained through the evaluation of the original staircase test. In summary, the modified staircase test introduces a colour-coded pellet system which obviously increases the test sensitivity and discloses new insights into the skilled forelimb use in a rat model of Parkinson's disease. It may therefore become a valuable tool in future studies related to plasticity-induced changes in skilled forelimb reaching and grasping movements. (c) 2006 Elsevier Inc. All rights reserved.