A METHOD FOR EVOKING PHYSIOLOGICAL-RESPONSES BY STIMULATION OF CELL-BODIES, BUT NOT AXONS OF PASSAGE, WITHIN LOCALIZED REGIONS OF THE CENTRAL NERVOUS-SYSTEM
A METHOD FOR EVOKING PHYSIOLOGICAL-RESPONSES BY STIMULATION OF CELL-BODIES, BUT NOT AXONS OF PASSAGE, WITHIN LOCALIZED REGIONS OF THE CENTRAL NERVOUS-SYSTEM
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DOI：
10.1016/0165-0270(82)90036-x
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发表时间：
1982-01-01
影响因子：
3
通讯作者：
BANDLER, R
中科院分区：
文献类型：
--
作者：
GOODCHILD, AK;DAMPNEY, RAL;BANDLER, R
本文介绍了一种将谷氨酸钠溶液微量注射到中枢神经系统局部区域引起生理反应的方法。这种方法的主要优点是，注射部位内的神经元的细胞体或树突状突起被激发，而通道轴突不受影响。将0.5 M谷氨酸溶液注射到麻醉或清醒家兔的髓质或中脑内的选定部位，分别引起明显的自主神经、躯体运动或行为反应，具体取决于注射部位。谷氨酸微量注射到纤维束未能引起任何反应，电刺激在相同的网站引起显着的反应。谷氨酸刺激的本地化程度和谷氨酸浓度和诱发反应的幅度之间的关系进行了描述。这种方法是选择性刺激中枢神经系统高度局部区域内细胞体的一种非常有效的手段。这种方法与局部电刺激相结合，使得在某些情况下有可能提供证据表明，反应是由通道轴突而不是细胞体的兴奋引起的。
A method for evoking physiological responses by microinjection of sodium glutamate solution into localized regions of the CNS was described. The major advantage of this method is that the cell bodies or dendritic processes of neurons within the injection site are excited, whereas axons of passage are unaffected. Injections of minute volumes (50-100 nl) of 0.5 M glutamate solution into selected sites within the medulla or midbrain of anesthetized or conscious rabbits, respectively, elicited marked autonomic, somatomotor or behavioral responses, depending on the injection site. Glutamate microinjection into fiber tracts failed to elicit any response; electrical stimulation at the same sites elicited marked responses. The degree of localization of the glutamate stimulus and the relation between glutamate concentration and magnitude of evoked response were described. This method was a very effective means of selectively stimulating cell bodies within highly localized regions of the CNS. This method in combination with focal electrical stimulation makes it possible in some cases to provide evidence that a response arises from excitation of axons of passage rather than cell bodies.