PURIFICATION OF HUMAN-IMMUNOGLOBULINS BY SEQUENTIAL PRECIPITATION WITH CAPRYLIC-ACID AND AMMONIUM-SULFATE
PURIFICATION OF HUMAN-IMMUNOGLOBULINS BY SEQUENTIAL PRECIPITATION WITH CAPRYLIC-ACID AND AMMONIUM-SULFATE
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DOI：
10.1016/0022-1759(90)90458-8
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发表时间：
1990-03-27
影响因子：
2.2
通讯作者：
DAMMACCO, F
中科院分区：
文献类型：
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作者：
PEROSA, F;CARBONE, R;DAMMACCO, F
我们已经测试了用辛酸和硫酸铵顺序沉淀法从11名单克隆丙种球蛋白病患者（4例IgG - κ型、2例IgG - λ型、2例IgM - κ型、1例IgA - κ型、2例IgA - λ型）、4名自身免疫性疾病患者和4名健康供体的血清中纯化人单克隆和多克隆免疫球蛋白的有效性。就免疫球蛋白的纯度和活性以及操作时间和成本而言，这种两步非色谱法对IgG、IgA和IgM的纯化非常高效，因此相较于其他纯化方法具有一些优势。所以，该方法在制备用于结构研究和治疗目的的人免疫球蛋白方面可能具有实用价值。
We have tested the usefulness of sequential precipitation with caprylic acid and ammonium sulfate to purify human monoclonal and polyclonal immunoglobulins from sera of 11 patients with monoclonal gammapathy (4 IgG.kappa., 2 IgG.lambda., 2 Igm.kappa., 1 IgA.kappa., 2 IgA.lambda.), four patients with autoimmune diseases and four healthy donors. In terms of purity and activity of Ig as well as execution time and cost, this two-step non-chromatographic procedure is highly efficient for the purification of IgG, IgA and IgM, thus offering several advantages over other methods of purification. Therefore, this procedure may have useful application in the preparation of human Ig for structural studies and therapeutic purposes.