Selection of optical biosensors from chemisynthetic antibody libraries
Selection of optical biosensors from chemisynthetic antibody libraries
复制标题
DOI：
10.1093/protein/gzh083
复制
发表时间：
2004-10-01
影响因子：
2.4
通讯作者：
Winter, G
中科院分区：
文献类型：
--
作者：
Jespers, L;Bonnert, TP;Winter, G
我们描述了一种方法来创建抗体与荧光报告整合到抗原结合位点。报告分子与噬菌体上显示的抗体库的高变环化学连接，该抗体库被选择用于抗原结合。在一种选定的抗体中，探针的荧光定量地响应抗原结合。该方法可应用于均相免疫分析的工程化。
We describe a method for creating antibodies with a fluorescent reporter integrated into the antigen-binding site. A reporter molecule was chemically linked to a hypervariable loop of an antibody repertoire displayed on phage, and this repertoire was selected for antigen binding. In one selected antibody, the fluorescence of the probe responded quantitatively to antigen binding. The method may have application for the engineering of homogeneous immunoassays.