An introduction to critical points for biophysicists; observations of compositional heterogeneity in lipid membranes.

An introduction to critical points for biophysicists; observations of compositional heterogeneity in lipid membranes.
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DOI:
10.1016/j.bbamem.2008.09.010
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发表时间:
2009-01
影响因子:
3.4
通讯作者:
Keller, Sarah L.
Keller, Sarah L.
中科院分区:
生物学3区
文献类型:
--
作者:
Honerkamp-Smith, Aurelia R.;Veatch, Sarah L.;Keller, Sarah L.

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与临界点相关的标度律有能力大大简化我们对复杂生物物理系统的描述。对于一般读者,我们首先回顾与一般读者的临界现象相关的基本概念和方程。然后,我们将这些概念应用到特定的生物物理系统的脂质膜。我们最近报道,脂膜可以包含组合物的波动行为的方式与二维伊辛普适类。在膜的临界点附近,这些波动是微米级的,可以通过荧光显微镜清楚地观察到。在较高的温度下,在临界点以上,我们希望找到亚微米的波动。在单独的工作中,我们已经报道了直接从细胞中分离的质膜表现出与模型膜相同的伊辛行为。我们回顾其他模型描述亚微米横向膜的不均匀性,包括微乳液,nanodomains,和平均场临界波动,我们描述的实验测试,可以区分这些模型。
Scaling laws associated with critical points have the power to greatly simplify our description of complex biophysical systems. For the general reader, we first review basic concepts and equations associated with critical phenomena for the general reader. We then apply these concepts to the specific biophysical system of lipid membranes. We recently reported that lipid membranes can contain composition fluctuations that behave in a manner consistent with the two-dimensional Ising universality class. Near the membrane’s critical point, these fluctuations are micron-sized, clearly observable by fluorescence microscopy. At higher temperatures, above the critical point, we expect to find submicron fluctuations. In separate work, we have reported that plasma membranes isolated directly from cells exhibit the same Ising behavior as model membranes do. We review other models describing submicron lateral inhomogeneity in membranes, including microemulsions, nanodomains, and mean field critical fluctuations, and we describe experimental tests that may distinguish these models.
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