A comparative analysis of cDNA-derived sequences for rat and mouse beta 3 integrins (GPIIIA) with their human counterpart.
A comparative analysis of cDNA-derived sequences for rat and mouse beta 3 integrins (GPIIIA) with their human counterpart.
复制标题
对大鼠和小鼠 β 3 整合素 (GPIIIA) 的 cDNA 衍生序列与其人类对应物进行比较分析。
DOI：
10.1006/bbrc.1993.1692
复制
发表时间：
1993
影响因子：
3.1
通讯作者：
Rifat,S
中科院分区：
文献类型：
--
作者：
Cieutat,AM;Rosa,JP;Letourneur,F;Poncz,M;Rifat,S
糖蛋白IIb受体3(αIIbβ3，GPIIb-IIIa)是整合素超家族的成员，是纤维蛋白原的血小板受体。我们现在已经克隆了小鼠和大鼠的β3cDNA。这些数据代表了第一批可用的非人类β3序列，使重要的比较分析成为可能。这两个β3序列都与人类β3高度同源，远高于啮齿动物与人类蛋白79%的平均同源性。配体结合区(AA)109-171和204-229)分别有90%和100%的同源性。β3跨膜和细胞质尾部的同源性高得令人惊讶，分别为97%和100%，但与β1或β2几乎没有同源性。后者的差异有力地支持了β3在这些结构域中的关键功能。总之，我们首次对β3链进行了比较分析，并证明了其总体同源性很高。讨论了这些比较的生物学意义。
αIIbβ3(GPIIb-IIIa), the platelet receptor for fibrinogen, is a member of the integrin superfamily. We have now cloned the mouse and rat β3cDNAs. These data represent the first available non-human β3sequences, allowing important comparative analyses. Both β3sequences are highly homologous with human β3, well above average rodent-human protein homology of 79%. The ligand binding domains (aa) 109-171 and 204-229) are,respectively, 90% and 100% homologous. The β3transmembrane and the cytoplasmic tail are surprisingly highly conserved, being 97% and 100% homologous, respectively, but share little homology with β1, or β2. This latter difference argues strongly in favor for a crucial β3-specific function for these domains. In conclusion, we present the first comparative analysis of β3chains and demonstrate high overall homology. The biological implications of these comparisons are discussed.