Insulin Release from Isolated Human Fetal Pancreatic Islets
Insulin Release from Isolated Human Fetal Pancreatic Islets
复制标题
离体人胎儿胰岛的胰岛素释放
作者：
A. E. D. L. Monteros;Shirley G. Driscoll;J. Steinke
胰腺获自5个12至16周龄的人胎儿。用胶原酶分离胰岛，然后用加入培养基的缓冲液、葡萄糖、甲苯磺丁脲或胰高血糖素孵育。通过免疫测定法测量释放到培养基中的胰岛素。胰高血糖素产生的唯一显著增加高于基线;葡萄糖和甲苯磺丁脲未能增强胰岛素分泌。这些数据表明，这个胎龄的分离的人胎儿胰岛对胰高血糖素的反应早于对葡萄糖或甲苯磺丁脲的反应。
Pancreases were obtained from five human fetuses 12 to 16 weeks old. The islets of Langerhans were isolated with collagenase, and then incubated with buffer, glucose, tolbutamide, or glucagon added to the medium. The insulin released into the medium was measured by immunoassay. Glucagon produced the only significant increase above base line; glucose and tolbutamide failed to enhance secretion of insulin. The data suggest that isolated human fetal islets of this gestational age develop responsiveness to glucagon earlier than to glucose or tolbutamide.