Distribution and cellular localization of actin depolymerizing factor.
Distribution and cellular localization of actin depolymerizing factor.
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DOI：
10.1083/jcb.105.6.2817
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发表时间：
1987-12
期刊：
影响因子：
--
通讯作者：
Bray D
中科院分区：
文献类型：
--
作者：
Bamburg JR;Bray D
肌动蛋白解聚因子（ADF）是一种低分子质量（19kd）的蛋白，与肌动蛋白形成紧密结合的二聚体复合体。用兔抗鸡脑ADF血清对鸡脑ADF的分布和细胞定位进行了分析。我们发现ADF是所有鸡胚和大多数成年鸡组织的主要成分，占总蛋白的0.1-0.4%。有些组织每摩尔肌动蛋白的ADF高达0.6 mol。成人心脏和骨骼肌的ADF水平非常低：低于0.02%的可溶性蛋白。在骨骼肌发育过程中，ADF水平在卵期11 d左右达到峰值，随后在产后14 d下降至成虫水平。来自其他几种脊椎动物（包括哺乳动物）的脑组织和培养细胞系都具有与ADF大小相同的蛋白质，这些蛋白质可被ADF抗血清识别。从棘阿米巴或几种无脊椎动物的神经组织中提取的ADF抗血清中没有特异性识别的蛋白质。间接免疫荧光显示ADF存在于大多数细胞的细胞质中、皱褶膜的前缘和神经元生长锥中。它的丰度和广泛分布，以及它隔离肌动蛋白分子的能力，即使是那些已经处于聚合状态的肌动蛋白分子，表明ADF是许多脊椎动物细胞中肌动蛋白丝调节的主要因素。
Actin depolymerizing factor (ADF) is a low molecular mass (19 kD) protein that forms a tightly bound dimeric complex with actin. We have raised a rabbit antiserum to chick brain ADF and used it to analyze the distribution and cellular localization of ADF. We find that ADF is a major constituent of all chick embryonic and most adult tissues examined, accounting for 0.1-0.4% of the total protein. Some tissues have as much as 0.6 mol ADF per mole actin. Adult heart and skeletal muscle are unusual in having very low levels of ADF: less than 0.02% of the soluble protein. During the development of skeletal muscle, ADF levels are maximal up to approximately 11 d in ovo and then decline to reach their adult levels by 14 d posthatching. Brain tissue and cultured cell lines from several other vertebrates, including mammals, all possess proteins of identical size to ADF that are recognized by the ADF antiserum. No proteins are specifically recognized by the ADF antiserum in extracts from Acanthamoeba castellanii or from nerve tissue of several invertebrates. Indirect immunofluorescence shows that ADF is present throughout the cytosol of most cells and at the leading edge of ruffled membranes and in the neuronal growth cone. Its abundance and widespread distribution together with its ability to sequester actin molecules, even those in an already polymerized state, suggest that ADF is a major factor in the regulation of actin filaments in many vertebrate cells.