The disturbance and clinical significance of B cell and circulating follicular helper T cell subsets in children with primary nephrotic syndrome
The disturbance and clinical significance of B cell and circulating follicular helper T cell subsets in children with primary nephrotic syndrome
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DOI：
10.1016/j.imlet.2021.07.006
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发表时间：
2021-07-30
影响因子：
4.4
通讯作者：
Li, Qiu
中科院分区：
文献类型：
--
作者：
Yang, Xia;Man, Changming;Li, Qiu
原发性肾病综合征的免疫发病机制尚不清楚。本研究通过流式细胞术检测循环B细胞和滤泡辅助性T细胞的频率、亚群和分子功能，并探讨新发患者、复发患者和健康对照中某些亚群与临床疾病指标的相关性。我们发现，在发病和复发患者中，CD86（+）活化细胞和CD19(+)CD138（+）浆细胞的比例增加。然而，仅在复发组中观察到CD27（+）记忆细胞百分比的增加。此外，ICOS MFI在新发患者的TFH细胞及其三个亚群中升高，而OX40在复发组的TFH细胞、TFH17细胞和TFH2细胞中表达升高。此外，在新发病组中，CD19(+)CD138（+）浆细胞频率的增加与尿素氮呈正相关。值得注意的是，CD86（+）活化的B细胞与CD19(+)CD138（+）浆细胞之间存在正相关，仅在新发病组和HC组存在；然而，只有复发组CD27（+）记忆细胞百分比的增加与24小时尿蛋白呈正相关。我们的研究结果表明，CD19(+)CD138（+）浆细胞可能是PNS发病的关键因素，而CD27（+）记忆细胞可能在PNS复发中发挥更重要的作用。此外，TFH细胞的功能也多种多样，因为在不同的疾病阶段，重要分子的表达会发生变化。
The immunopathogenesis of primary nephrotic syndrome is unclear. Here, we examined the frequency, subsets and molecular function of circulating B cells and follicular helper T cells by flow cytometry and explored the correlation between certain subsets and clinical disease indices in new-onset patients, relapsing patients and healthy controls. We found an increase in the proportions of CD86(+) activated cells and CD19(+)CD138(+) plasma cells in the patients at onset and patients in relapse. However, the increased percentage of CD27(+) memory cells was observed in only the relapse group. Furthermore, the ICOS MFI was elevated in TFH cells and their three subsets in new-onset patients, whereas the expression of OX40 was increased in TFH cells, TFH17 cells and TFH2 cells in the relapse group. Additionally, the increased frequency of CD19(+)CD138(+) plasma cells was positively associated with urea nitrogen in the new-onset groups. Notably, the positive correlation between CD86(+) activated B cells and CD19(+)CD138(+) plasma cells was obtained in only the new-onset group and HC group; however, the increased CD27(+) memory cell percentage was positively correlated with 24-h urinary protein in only the relapse group. Our results indicate that CD19(+)CD138(+) plasma cells may be a key factor for the onset of PNS, whereas CD27(+) memory cells may play a more important role in the relapse of this disease. Furthermore, the functions of TFH cells are also diverse because the expression of vital molecules changes during the different disease phases.