ClearT: a detergent- and solvent-free clearing method for neuronal and non-neuronal tissue
ClearT: a detergent- and solvent-free clearing method for neuronal and non-neuronal tissue
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DOI：
10.1242/dev.091844
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发表时间：
2013-03-01
期刊：
影响因子：
4.6
通讯作者：
Mason, Carol
中科院分区：
文献类型：
--
作者：
Kuwajima, Takaaki;Sitko, Austen A.;Mason, Carol
我们描述了一种透明化方法，用于增强神经元和非神经元组织中细胞形态及连接的可视化效果。使用分别由甲酰胺或甲酰胺/聚乙二醇组成的Clear(T)或Clear(T2)，胚胎、整装标本以及厚脑切片能够在体积变化极小的情况下被快速透明化。与其他现有的透明化技术不同，这些方法不使用洗涤剂或溶剂，因此能保留亲脂性染料、荧光示踪剂和免疫组织化学标记以及荧光蛋白标记。
We describe a clearing method for enhanced visualization of cell morphology and connections in neuronal and non-neuronal tissue. Using Clear(T) or Clear(T2), which are composed of formamide or formamide/polyethylene glycol, respectively, embryos, whole mounts and thick brain sections can be rapidly cleared with minimal volume changes. Unlike other available clearing techniques, these methods do not use detergents or solvents, and thus preserve lipophilic dyes, fluorescent tracers and immunohistochemical labeling, as well as fluorescent-protein labeling.