TEMPORALLY DISTINCTIVE CHANGES OF ALTERNATIVE SPLICING PATTERNS DURING MYOGENIC DIFFERENTIATION OF C2C12 CELLS
TEMPORALLY DISTINCTIVE CHANGES OF ALTERNATIVE SPLICING PATTERNS DURING MYOGENIC DIFFERENTIATION OF C2C12 CELLS
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DOI：
10.1093/oxfordjournals.jbchem.a124980
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发表时间：
1995-10-01
影响因子：
2.7
通讯作者：
ASANO, A
中科院分区：
文献类型：
--
作者：
HARADA, Y;NAKAMURA, M;ASANO, A
众所周知，骨骼肌分化伴随着许多肌肉特异性成分的出现，其中一些成分是通过肌肉特异性选择性剪接产生的，但目前尚不清楚在肌源性分化过程中，调控肌肉特异性剪接反应的系统是以何种方式(包括时间)构建的。我们同时利用逆转录-聚合酶链式反应方法检测了神经细胞黏附分子(NCAM)、β-原肌球蛋白和M型丙酮酸激酶基因在培养成肌细胞分化过程中几种剪接模式的变化。诱导分化后，NCAM糖基磷脂酰肌醇锚定形式优先于跨膜形式增加，而NCAM MSD1(肌肉特异性结构域1)外显子的选择在细胞融合出现时开始并突然增加。在C2C12细胞的成肌分化过程中，这些基因的每一个转录本都独立地表现出不同的亚型选择变化规律。这些观察表明，在肌源性分化过程中，选择性剪接反应应该发生一些独立的调节。
It is well known that skeletal muscle differentiation is accompanied by the appearance of many muscle-specific components and that some of these components are generated through muscle-specific alternative splicing, It is not clear, however, in what manner, including timing, the system that regulates the muscle-specific splicing reactions is constructed during the process of myogenic differentiation. We simultaneously examined the changes in several splicing patterns for the neural cell adhesion molecule (NCAM), beta-tropomyosin, and M-type pyruvate kinase genes during myogenic differentiation of cultured myoblasts using the reverse transcription-polymerase chain reaction method. The NCAM glycosylphosphatidylinositol anchor form increased in preference to the transmembrane form immediately after the induction of differentiation, while the selection of NCAM MSD1 (muscle-specific domain 1) exons started and abruptly increased at about the time when cell-fusion appeared. M2-type pyruvate kinase was gradually substituted for the M1-type molecule, Skeletal muscle-type beta-tropomyosin was predominantly selected even in myoblasts in the growth medium, As a result, each transcript of these genes independently showed a temporally distinctive pattern of change in isoform selection during the myogenic differentiation of C2C12 cells. These observations suggest that some independent regulation of alternative splicing reactions should occur during myogenic differentiation.