Expression and developmental function of the 3-ketoacyl-ACP synthase2 gene in Arabidopsis thaliana.
Expression and developmental function of the 3-ketoacyl-ACP synthase2 gene in Arabidopsis thaliana.
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DOI：
10.1266/ggs.83.143
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发表时间：
2008-04
影响因子：
1.1
通讯作者：
Hirokazu Hakozaki;Jong-In Park;M. Endo;Y. Takada;T. Kazama;Yoshimitsu Takeda;G. Suzuki;M. Kawagishi-Kobayashi;Masao Watanabe
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文献类型：
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作者：
Hirokazu Hakozaki;Jong-In Park;M. Endo;Y. Takada;T. Kazama;Yoshimitsu Takeda;G. Suzuki;M. Kawagishi-Kobayashi;Masao Watanabe
3-酮酰基-ACP 合酶 (KAS) II 是一种脂肪酸相关酶，可催化质体中 16:0-酰基载体蛋白 (ACP) 延伸至 18:0-ACP。拟南芥脂肪酸生物合成1-1（fab1-1）突变体部分缺乏拟南芥3-酮酰基-ACP合酶2（AtKAS2）的活性，其表型与抗冷性和脂肪酸组成的关系已得到深入研究。在本研究中，我们使用 AtKAS2 的 T-DNA 插入突变体来研究其在植物发育中的可能作用。逆转录(RT)-PCR表明AtKAS2基因在除根外的多种植物器官中表达，并且在长角果中高表达。 β-葡萄糖醛酸酶 (GUS) 与 AtKAS2 启动子的融合表明该启动子在胚胎、气孔保卫细胞、花序和花粉粒等多种组织中具有活性。我们无法在杂合突变体后代中鉴定出 atkas2 纯合突变体成年植物。表型和遗传分析表明，T-DNA 插入对 AtKAS2 的破坏导致胚胎致死，并且胚胎的发育在球形阶段停止。综上所述，我们的结果表明，AtKAS2 是拟南芥从球状阶段到心状阶段的胚胎发育所必需的。
The 3-ketoacyl-ACP synthase (KAS) II is a fatty-acid-related enzyme which catalyzes the elongation of 16:0-acyl carrier protein (ACP) to 18:0-ACP in plastids. The fatty acid biosynthesis 1-1 (fab1-1) mutant of Arabidopsis thaliana is partially deficient in its activity of Arabidopsis thaliana 3-ketoacyl-ACP synthase 2 (AtKAS2), and its phenotype has been intensively studied in connection with the chilling resistance and fatty acid composition. In this study, we used the T-DNA insertion mutant of AtKAS2 to examine its possible role in plant development. Reverse transcription (RT)-PCR showed that the AtKAS2 gene was expressed in various plant organs, except for roots, and was highly expressed in siliques. The fusion of beta-glucuronidase (GUS) to the AtKAS2 promoter demonstrated that the promoter was active in various tissues such as embryos, stomatal guard cells, inflorescences and pollen grains. We were not able to identify atkas2 homozygous mutant adult plants in heterozygous mutant progeny. Phenotypic and genetic analyses showed that disruption of the AtKAS2 by T-DNA insertion caused embryo lethality, and the development of the embryos was arrested at the globular stage. Taken together, our results suggest that AtKAS2 is required for embryo development in Arabidopsis during the transition from the globular to the heart stage.