Role of MicroRNA-214 in ginsenoside-Rg1-induced angiogenesis
Role of MicroRNA-214 in ginsenoside-Rg1-induced angiogenesis
复制标题
DOI：
10.1016/j.ejps.2009.08.008
复制
发表时间：
2009-11-05
影响因子：
4.6
通讯作者：
Wong, Ricky Ngok-Shun
中科院分区：
文献类型：
--
作者：
Chan, Lai-Sheung;Yue, Patrick Ying-Kit;Wong, Ricky Ngok-Shun
microRNA（miRNAs）是一类小的非编码RNA，是转录后基因调控因子。人参皂甙Rg 1是人参的活性成分之一，已被证实具有血管生成诱导剂的作用。使用miRNA微阵列分析。在人脐静脉内皮细胞（HUVECs）中，Rg 1分别下调或上调了17种（包括miR-214）和5种miRNAs。由于miR-214与内皮型一氧化氮合酶（eNOS）密切相关，因此与血管生成密切相关，因此通过qRT-PCR进一步验证了其表达。我们还通过转染特异性miRNA抑制剂或前体研究了miR-214对内皮型一氧化氮合酶表达的作用，以及在HUVEC小管形成和运动中的作用。我们的研究结果表明，Rg 1可以下调HUVEC中miR-214的表达，导致eNOS表达增加，并在体外细胞迁移和管形成，这可能是促进血管生成。这些结果意味着对简单的天然化合物如何通过调节miRNA表达来影响生理变化的新理解。(C)2009 Elsevier B. V.保留所有权利。
MicroRNAs (miRNAs) are small non-coding RNAs that act as post-transcriptional gene modulators. Ginsenoside-Rg1, one of the active components of ginseng, has been confirmed as an angiogenesis inducer. Using miRNA microarray analysis. a total of 17 (including miR-214) and 5 miRNAs were found to be down- or up-regulated by Rg1 in human umbilical vein endothelial cells (HUVECs), respectively. Since miR-214 is closely related to endothelial nitric oxide synthase (eNOS) and hence angiogenesis, its expression was further validated by qRT-PCR. We also investigated the role of miR-214 on eNOS expression and in tubulogenesis and motility of HUVEC by transfection of specific miRNA inhibitor or precursor. Our results suggested that Rg1 can down-regulate miR-214 expression in HUVEC, leading to an increase in eNOS expression, and in vitro cell migration and tube formation which can possibly promote angiogenesis. These results signify a new understanding towards how a simple natural compound can affect physiological changes through modulation of miRNA expression. (C) 2009 Elsevier B.V. All rights reserved.