SACCHAROMYCES-CEREVISIAE SPT3 GENE IS REQUIRED FOR TRANSPOSITION AND TRANSPOSITIONAL RECOMBINATION OF CHROMOSOMAL TY ELEMENTS
SACCHAROMYCES-CEREVISIAE SPT3 GENE IS REQUIRED FOR TRANSPOSITION AND TRANSPOSITIONAL RECOMBINATION OF CHROMOSOMAL TY ELEMENTS
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DOI：
10.1128/mcb.6.11.3575
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发表时间：
1986-11-01
影响因子：
5.3
通讯作者：
FINK, GR
中科院分区：
文献类型：
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作者：
BOEKE, JD;STYLES, CA;FINK, GR
酿酒酵母SPT 3基因的突变对Ty元件和与该元件相邻的基因的表达具有显著影响。SPT 3基因对于Ty转座是必需的，因为当SPT 3基因被移码突变spt 3 -101替换时，染色体Ty元件的转座停止。据推测，转座的消除是由于SPT 3基因产物对Ty转录的影响;在spt 3 -101菌株中，染色体Ty元件的转录物大部分被消除（F. Winston，K. J. Durbin和G. R. Fink，Cell 39：675-682，1984）。在spt 3 -101菌株中的Ty转录可以通过引入pGTyH 3质粒来重建，其中Ty元件TyH 3的转录在GAL 1启动子的控制下;这些质粒衍生的Ty转录物是SPT 3-独立的。在含有pGTyH 3质粒的spt 3 -101菌株中，半乳糖诱导后Ty转座恢复。在spt 3突变体中，几乎所有产生的转座事件都源自pGTyH 3质粒，而不是染色体元件。
Mutations in the Saccharomyces cerevisiae SPT3 gene have dramatic effects on the expression of Ty elements and genes adjacent to the element. The SPT3 gene is essential for Ty transposition, because transposition of chromosomal Ty elements ceased when the SPT3 gene was replaced with the frameshift mutation spt3-101. Presumably, the elimination of transposition was due to the effect of the SPT3 gene product on Ty transcription; the transcripts of chromosomal Ty elements were largely abolished in the spt3-101 strain (F. Winston, K. J. Durbin, and G. R. Fink, Cell 39:675-682, 1984). Ty transcription in an spt3-101 strain could be reestablished by introduction of the pGTyH3 plasmid, in which transcription of the Ty element TyH3 is under the control of the GAL1 promoter; these plasmid-derived Ty transcripts were SPT3-independent. Ty transposition resumed after galactose induction in spt3-101 strains containing the pGTyH3 plasmid. In spt3 mutants nearly all of the resulting transposition events derived from pGTyH3 plasmids and not from chromosomal elements.