Expression of ClPAP and ClPSY1 in watermelon correlates with chromoplast differentiation, carotenoid accumulation, and flesh color formation
Expression of ClPAP and ClPSY1 in watermelon correlates with chromoplast differentiation, carotenoid accumulation, and flesh color formation
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DOI：
10.1016/j.scienta.2020.109437
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发表时间：
2020-08-25
影响因子：
4.3
通讯作者：
Dai, Zuyun
中科院分区：
文献类型：
--
作者：
Fang, Xufeng;Liu, Shi;Dai, Zuyun
用光学显微镜和透射电子显微镜观察了不同西瓜品种果肉发育过程中的染色体分化。类胡萝卜素的LC-MS/MS分析表明，红肉西瓜在成熟时主要积累番茄红素，最终形成结晶状的有色体，而其他类胡萝卜素的积累导致球状有色体的形成。分析了3个植酸合成酶基因(GlPSY)和7个叶绿体脂肪相关基因(ClPAP)在不同肉质发育阶段的表达。根据我们的结果，ClPSYI的表达与植物荧烯和总类胡萝卜素的积累一致，而ClPAP(Cla014416)的表达与ClPSYI的表达一致。ClPSYI和Cla014416的CDS和启动子序列变异可能是基于基因和启动子克隆的显著表达水平的主要原因。
Chromoplast differentiation during flesh development in different watermelon varieties was observed by optical and transmission electron microscopy. Carotenoid analyses by LC-MS/MS revealed that red-fleshed watermelon mainly accumulated lycopene, which eventually shaped into a crystalloid chromoplast near maturation, while the accumulation of other carotenoids led to the formation of globular chromoplasts. Expressions of three phytoene synthase genes (GlPSY) and seven plastid lipid-associated genes (ClPAP) in different flesh development stages were analyzed. Based on our results, the expression of ClPSYI was consistent with the accumulation of phytofluene and total carotenoids, while that of ClPAP (Cla014416) was consistent with ClPSYI. CDS and promoter sequence variations of ClPSYI and Cla014416 may be the main reason for the significant expression levels based on gene and promoter cloning.