Effects of enamel matrix derivative on mineralized tissue formation during bone wound healing in rat parietal bone defects
Effects of enamel matrix derivative on mineralized tissue formation during bone wound healing in rat parietal bone defects
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DOI：
10.1093/jmicro/51.6.413
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发表时间：
2002-01-01
期刊：
影响因子：
--
通讯作者：
Sasaki, T
中科院分区：
文献类型：
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作者：
Sawae, Y;Sahara, T;Sasaki, T
釉质基质衍生物（EMD：Emdogain（C））已被报道刺激骨小梁的生物合成和再生。为了解决EMD的生物学作用是否依赖于骨组织的局部环境，在顶骨中制造圆形穿孔，并立即填充EMD或其载体藻酸丙二醇酯（PGA）作为对照。在术后第4-60天，通过各种组织学技术检查解剖的骨。新骨基质，这是骨唾液酸蛋白（BSP）的免疫反应，从周围地区的穿孔骨膜形成。与在受伤的长骨中报道的结果不同，在骨缺损内的再生结缔组织中产生矿化组织。这种矿化组织几乎没有BSP免疫染色，含有少量胶原纤维，缺乏骨细胞陷窝和成骨细胞层和类骨质。能量色散X射线分析表明，钙和磷的重量%和钙/磷摩尔比的矿化组织类似于或略高于先前存在的顶骨。此外，大多数位于矿化组织中的多核细胞缺乏皱褶边缘结构，对溶酶体半胱氨酸蛋白酶组织蛋白酶K的免疫反应较弱，而位于骨基质中的多核细胞则表现出皱褶边缘和强组织蛋白酶K表达。然而，位于这两种组织中的多核细胞强烈染色的抗酒石酸酸性磷酸酶。这种矿化组织的体积分数似乎是较高的EMD应用骨比PGA应用的控制。骨缺损内的矿化组织形成基质细胞在EMD应用的骨中比PGA应用的对照中显示出更大的积累。我们的研究结果表明，EMD对骨创伤愈合和矿化组织形成的生物活性作用至少部分取决于EMD应用的局部骨环境。
Enamel matrix derivative (EMD: Emdogain(C)) has been reported to stimulate the biosynthesis and regeneration of trabecular bone. To address whether the biological action of EMD is dependent on the local environment of osseous tissue, circular perforations were made in parietal bones and immediately filled with either EMD or its carrier, propylene glycol alginate (PGA), as control. On post-operative days 4-60, the dissected bones were examined by various histological techniques. New bone matrix, which was immunoreactive for bone sialoprotein (BSP), was formed from the periosteum at the peripheral area of perforations. Different from the findings reported in injured long bones, mineralized tissue was produced in the regenerating connective tissue within bone defects. This mineralized tissue was hardly immunostained for BSP, contained few collagen fibres, and lacked osteocytic lacunae and layers of osteoblasts and osteoid. Energy-dispersive X-ray analysis showed that Ca and P weight % and Ca/P molar ratio of this mineralized tissue were similar to or slightly higher than those in the pre-existing parietal bones. In addition, most multinucleated cells located in mineralized tissue lacked a ruffled border structure and showed weak inummoreaction for the lysosomal cysteine proteinase, cathepsin K, whereas those located in the bone matrix exhibited ruffled borders and strong cathepsin K expression. However, multinucleated cells located in both tissues were strongly stained for tartrate-resistant acid phosphatase. The volume fraction of such mineralized tissue appeared to be higher in EMD-applied bones than in PGA-applied controls. The mineralized tissue-forming stromal cells within bone defects appeared to show greater accumulation in EMD-applied bones than in PGA-applied controls. Our results suggest that the bioactive effects of EMD on bone wound healing and mineralized tissue formation depend, at least in part, on the local osseous environment where EMD has been applied.