CHOLESTEROL EFFLUX FROM CULTURED ADIPOSE-CELLS IS MEDIATED BY LPAI PARTICLES BUT NOT BY LPAI-AII PARTICLES
CHOLESTEROL EFFLUX FROM CULTURED ADIPOSE-CELLS IS MEDIATED BY LPAI PARTICLES BUT NOT BY LPAI-AII PARTICLES
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DOI：
10.1016/0006-291x(87)90451-7
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发表时间：
1987-01-15
影响因子：
3.1
通讯作者：
AILHAUD, G
中科院分区：
文献类型：
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作者：
BARBARAS, R;PUCHOIS, P;AILHAUD, G
在培养的脂肪细胞中研究了胆固醇流出后预加载LDL胆固醇。长期暴露于LPAI和LpAI：从HDL组分中分离的AII颗粒表明，LpAI颗粒仅能够促进胆固醇流出。测试了含有不同ApoAI/ApoAII摩尔比的脂质体：ApoAI的比例越大，从Ob 1771细胞中去除胆固醇的能力越快。剂量-反应曲线显示，LpAI颗粒在生理浓度范围内具有活性，而LpAI：AII颗粒在所有浓度下均无作用。结果是有利于LpAI颗粒的HDL组分的活性成分，促进胆固醇流出，并表明，LpAI颗粒和LpAI：AII颗粒代表不同的代谢实体。
Cholesterol efflux was studied in cultured adipose cells after preloading with LDL cholesterol. Long-term exposure to LPAI and LpAI:AII particles isolated from the HDL fraction showed that LpAI particles only were able to promote cholesterol efflux. Liposomes containing different ApoAI/ApoAII molar ratios were tested: the larger the proportion of ApoAI, the faster the ability to remove cholesterol from Ob1771 cells. Dose-response curves showed that LpAI particles were active within a physiological range of concentrations, whereas LpAI:AII particles had no effect at all concentrations. The results are in favour of LpAI particles being the active components of the HDL fraction for the promotion of cholesterol efflux and suggest that LpAI particles and LpAI:AII particles represent distinct metabolic entities.