Determination of catalase, peroxidase, and superoxide dismutase within the genus Legionella
Determination of catalase, peroxidase, and superoxide dismutase within the genus Legionella
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军团菌属内过氧化氢酶、过氧化物酶和超氧化物歧化酶的测定
DOI：
10.1128/jcm.20.3.421-429.1984
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发表时间：
1984
影响因子：
9.4
通讯作者：
M. Keen
中科院分区：
文献类型：
--
作者：
L. Pine;P. Hoffman;G. Malcolm;R. Benson;M. Keen
我们研究了40株军团菌的还原氧清除酶。使用一个简单的反应室与Swinney过滤器的啤酒和Sizer测定，我们确定了过氧化氢酶活性的活细胞上生长的缓冲木炭酵母提取物琼脂。对于29株嗜肺军团菌，过氧化氢酶的表观一级速率常数范围为0.000至0.005。同样，观察到瓦氏军团菌、奥克里根军团菌和戈氏军团菌的低值范围为0.001 - 0.005。高过氧化氢酶的活性被发现为Legionella jordanis，Legionella longbeachae，Legionella micdadei和Legionella bozemanii，一阶速率常数值为0.010至0.035。分析无细胞提取物的过氧化氢酶，过氧化物酶和超氧化物歧化酶。所有菌株的无细胞提取物的超氧化物歧化酶水平范围从8.2至30.5 U每毫克蛋白质。过氧化氢酶和过氧化物酶是该种的特征酶。pneumophila和L. gormanii只有过氧化物酶（相对分子质量[Mr]为150，000）; dumoffii有一个过氧化物酶（Mr，150，000）和一个过氧化氢酶（Mr，174，000）;所有其余的物种只有过氧化氢酶（Mr，300，000，220，000或150，000）。
We examined 40 strains of Legionella for reduced-oxygen scavenging enzymes. Using a simple reaction chamber with a Swinney filter for the Beers and Sizer assay, we determined the catalase activity of live cells grown on buffered charcoal-yeast extract agar. For 29 strains of Legionella pneumophila, the apparent first-order rate constants for catalase ranged from 0.000 to 0.005. Similarly, low values ranging from 0.001 to 0.005 were observed for Legionella wadsworthii, Legionella oakridgensis, and Legionella gormanii. High catalase activities were found for Legionella jordanis, Legionella longbeachae, Legionella micdadei, and Legionella bozemanii, with first-order rate constant values of 0.010 to 0.035. Cell-free extracts were analyzed for catalase, peroxidase, and superoxide dismutase. Cell-free extracts of all strains had superoxide dismutase levels ranging from 8.2 to 30.5 U per mg of protein. The species could be characterized by their catalase and peroxidase since L. pneumophila and L. gormanii had only peroxidase (relative molecular weight [Mr], 150,000); L. dumoffii had a peroxidase (Mr, 150,000) plus a catalase (Mr, 174,000); and all remaining species had catalase only (Mr, 300,000, 220,000, or 150,000).
嗜肺军团菌对氧依赖性杀菌系统的敏感性。
DOI：
--
发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Locksley,RM;Jacobs,RF;Wilson,CB;Weaver,WM;Klebanoff,SJ
通讯作者：
Klebanoff,SJ