Distinctive profile of monomeric and polymeric anti-SSA/Ro52 immunoglobulin A1 isoforms in saliva of patients with primary Sjögren's syndrome and Sicca.
Distinctive profile of monomeric and polymeric anti-SSA/Ro52 immunoglobulin A1 isoforms in saliva of patients with primary Sjögren's syndrome and Sicca.
复制标题
原发性干燥综合征和干燥症患者唾液中单体和聚合抗 SSA/Ro52 免疫球蛋白 A1 亚型的独特特征。
DOI：
10.1136/rmdopen-2023-003666
复制
发表时间：
2024
期刊：
影响因子：
6.2
通讯作者：
Chia,David
中科院分区：
文献类型：
--
作者：
Chiang,Samantha;Grogan,Tristan;Kamounah,Sarah;Wei,Fang;Tayob,Nabihah;Kim,JuYeon;KyunPark,Jin;Akin,David;Elashoff,DavidA;Pedersen,AnneMarieLynge;Song,YeongWook;Wong,DavidTW;Chia,David
目的原发性干燥综合征（pSS）是第二常见的慢性自身免疫性结缔组织病。血清中的自身抗体，免疫球蛋白（IgG）抗SSA/Ro，是pSS的关键诊断特征。由于pSS是一种唾液腺疾病，我们研究了唾液中的抗SSA/Ro 52抗体。方法利用一种新的电化学检测平台，电场诱导释放和测量，我们检测了IgG/M/A，IgG，伊加，伊加同种型（IgA 1和IgA 2）和IgA 1亚类34例pSS、35例干眼症和口干症患者的唾液上清液中的抗SSA/Ro 52抗体（聚合和单体IgA 1）结果唾液IgG/M/A、IgG、伊加、伊加同种型及抗SSA/Ro 52的IgA 1亚类在pSS、非pSS Sicca组和健康对照组之间差异有显著性。在非pSS Sicca患者中观察到单体IgA 1升高，而在pSS患者中观察到聚合IgA 1（pIgA 1）升高。唾液多聚体而非单体IgA 1（mIgA 1）亚型与病灶评分相关（r2=0.467，p=0.001）。结论唾液抗Ro 52多聚体IgA 1亚型与pSS的腺体炎症相关，而唾液单体IgA 1与Sicca相关。IgA 1同位素转换是否在Sicca到pSS的进展中起作用，需要进一步研究。
ObjectivePrimary Sjögren’s syndrome (pSS) is the second most common chronic autoimmune connective tissue disease. Autoantibodies, immunoglobulin (IgG) anti-SSA/Ro, in serum is a key diagnostic feature of pSS. Since pSS is a disease of the salivary gland, we investigated anti-SSA/Ro52 in saliva.MethodsUsing a novel electrochemical detection platform, Electric Field-Induced Release and Measurement, we measured IgG/M/A, IgG, IgA, IgA isotypes (IgA1 and IgA2) and IgA1 subclasses (polymeric and monomeric IgA1) to anti-SSA/Ro52 in saliva supernatant of 34 pSS, 35 dry eyes and dry mouth (patients with Sicca) and 41 health controls.ResultsSaliva IgG/M/A, IgG, IgA, IgA isotypes and IgA1 subclasses to anti-SSA/Ro52 differed significantly between pSS, non-pSS Sicca and healthy subjects. Elevated monomeric IgA1 was observed in patients with non-pSS Sicca while elevated polymeric IgA1 (pIgA1) was observed in patients with pSS. Salivary polymeric but not monomeric IgA1 (mIgA1) isoform correlated with focus score (r2=0.467, p=0.001)ConclusionsSalivary anti-Ro52 polymeric IgA1 isoform is associated with glandular inflammation in pSS, while salivary monomeric IgA1 is associated with Sicca. Whether IgA1 isotope switching plays a role in the progression of the Sicca to pSS warrants further investigation.