CDC25: a component of the RAS-adenylate cyclase pathway in Saccharomyces cerevisiae.
CDC25: a component of the RAS-adenylate cyclase pathway in Saccharomyces cerevisiae.
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DOI：
10.1126/science.3547648
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发表时间：
1987-03
期刊：
影响因子：
56.9
通讯作者：
L. C. Robinson;J. Gibbs;M. Marshall;I. Sigal;K. Tatchell
中科院分区：
文献类型：
--
作者：
L. C. Robinson;J. Gibbs;M. Marshall;I. Sigal;K. Tatchell
酿酒酵母含有ras致癌基因家族的两个功能同源物RAS1和RAS2。这些基因是生长所必需的，所有证据表明，这种基本功能是腺苷酸环化酶的激活。相反，哺乳动物细胞中的ras似乎不影响腺苷酸环化酶的活性。为了阐明ras在酵母和高等真核生物中的功能关系，以及酵母ras在生长控制中的作用，有必要确定ras在腺苷酸环化酶途径上游的功能。这里提出的证据表明，CDC25，由条件细胞周期阻滞突变鉴定，编码这样的上游功能。
The yeast Saccharomyces cerevisiae contains two functional homologues of the ras oncogene family, RAS1 and RAS2. These genes are required for growth, and all evidence indicates that this essential function is the activation of adenylate cyclase. In contrast, ras in mammalian cells does not appear to influence adenylate cyclase activity. To clarify the relation between ras function in yeast and in higher eukaryotes, and the role played by yeast RAS in growth control, it is necessary to identify functions acting upstream of RAS in the adenylate cyclase pathway. The evidence presented here indicates that CDC25, identified by conditional cell cycle arrest mutations, encodes such an upstream function.