The spatiotemporal expression pattern of the bone morphogenetic protein family in rat ovary cell types during the estrous cycle.
The spatiotemporal expression pattern of the bone morphogenetic protein family in rat ovary cell types during the estrous cycle.
复制标题
在发情周期中，大鼠卵巢细胞类型中骨形态发生蛋白家族的时空表达模式。
DOI：
10.1186/1477-7827-1-9
复制
发表时间：
2003-02-05
影响因子：
4.4
通讯作者：
Shimasaki, Shunichi
中科院分区：
文献类型：
--
作者：
Erickson, Gregory F;Shimasaki, Shunichi
在哺乳动物卵巢中，骨形态发生蛋白(BMP)家族的表达和功能最近引起了人们的极大兴趣，因为有证据表明，BMP家族在决定卵泡发生和女性生育能力方面发挥着关键作用。尽管有大量的工作，但仍需要了解这些重要调控分子的细胞表达部位，以及它们的基因表达如何在基本卵巢细胞类型中通过周期发生变化。在此，我们对BMP配体(BMP-2，-3，-3b，-4，-6，-7，-15)、受体(BMPR-IA，-IB，-II)和BMP拮抗剂卵泡抑素在正常发情周期大鼠卵巢中的时空表达模式进行了详细的原位杂交分析。我们发现：i)所有的mRNAs都以细胞特异性的方式在主要类型的卵巢细胞(卵母细胞、颗粒细胞、间质膜、外膜、黄体、次级间质、血管和卵巢表面上皮)中表达；ii)大多数在卵泡和黄体的形态发生和组织发生过程中发生动态变化。从这些研究中得出的一般原则是，卵泡发生(招募、选择、闭锁)、排卵和黄体发生(黄体化、黄体溶解)的发育程序伴随着这些BMP基因表达模式的相当戏剧性的时空变化。这些结果使我们假设BMP家族在决定基本发育事件中扮演了以前未曾预料到的角色，这些事件确保了发情周期产生所需的适当时间和发育事件。
In the mammalian ovary, great interest in the expression and function of the bone morphogenetic protein (BMP) family has been recently generated from evidence of their critical role in determining folliculogenesis and female fertility. Despite extensive work, there is a need to understand the cellular sites of expression of these important regulatory molecules, and how their gene expression changes within the basic ovary cell types through the cycle. Here we have performed a detailed in situ hybridization analysis of the spatial and temporal expression patterns of the BMP ligands (BMP-2, -3, -3b, -4, -6, -7, -15), receptors (BMPR-IA, -IB, -II), and BMP antagonist, follistatin, in rat ovaries over the normal estrous cycle. We have found that: i) all of the mRNAs are expressed in a cell-specific manner in the major classes of ovary cell types (oocyte, granulosa, theca interstitial, theca externa, corpora lutea, secondary interstitial, vascular and ovary surface epithelium); and ii) most undergo dynamic changes during follicular and corpora luteal morphogenesis and histogenesis. The general principle to emerge from these studies is that the developmental programs of folliculogenesis (recruitment, selection, atresia), ovulation, and luteogenesis (luteinization, luteolysis) are accompanied by rather dramatic spatial and temporal changes in the expression patterns of these BMP genes. These results lead us to hypothesize previously unanticipated roles for the BMP family in determining fundamental developmental events that ensure the proper timing and developmental events required for the generation of the estrous cycle.