LOWRY PROTEIN DETERMINATION ON MEMBRANE PREPARATIONS - NEED FOR STANDARDIZATION BY AMINO-ACID-ANALYSIS
LOWRY PROTEIN DETERMINATION ON MEMBRANE PREPARATIONS - NEED FOR STANDARDIZATION BY AMINO-ACID-ANALYSIS
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DOI：
10.1016/0003-2697(82)90050-1
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发表时间：
1982-01-01
影响因子：
2.9
通讯作者：
BONTING, SL
中科院分区：
文献类型：
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作者：
PETERS, WHM;FLEURENJAKOBS, AMM;BONTING, SL
以牛血清白蛋白为标准，定量氨基酸分析为绝对法，采用Lowry法测定3种膜蛋白制剂（兔肾和猪胃粘膜atp酶、牛视紫红质）的蛋白质含量。结果差别很大，Lowry方法给出的值高出29-42%。这意味着许多关于这类蛋白质的公开数据，基于以牛血清白蛋白作为普遍应用标准的Lowry蛋白测定，是错误的。对如何规范Lowry法，使膜蛋白的测定值更可靠提出了建议。
The protein content of 3 membrane protein preparations [rabbit kidney and porcine gastric mucosa ATPases, bovine rhodopsin] was determined by the Lowry method with bovine serum albumin as a standard as well as by quantitative amino acid analysis as an absolute method. The results differ considerably, the Lowry method giving 29-42% higher values. This implies that many published data for such proteins, based on Lowry protein determinations with bovine serum albumin as the generally applied standard, are in error. Suggestions are made on how to standardize the Lowry method so that reliable values can be obtained for membrane protein.