Application of quantitative gene expression analysis for pertussis vaccine safety control
Application of quantitative gene expression analysis for pertussis vaccine safety control
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DOI：
10.1016/j.vaccine.2008.06.086
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发表时间：
2008-08-26
期刊：
影响因子：
5.5
通讯作者：
Yamaguchi, Kazunari
中科院分区：
文献类型：
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作者：
Hamaguchi, Isao;Imai, Jun-ichi;Yamaguchi, Kazunari
尽管疫苗常用于预防传染病，但人们对疫苗造成的综合影响知之甚少。在这项研究中，我们通过综合基因表达分析表明，百日咳疫苗影响了疫苗治疗动物多个器官中的许多基因。特别是，肺被认为是评估百日咳疫苗毒性的最合适靶点。第一天对经过疫苗处理的肺部进行分析，鉴定出 13 个基因，显示出与百日咳疫苗毒性相对应的清晰树状图。此外，对这些基因的定量分析揭示了它们各自的表达水平与在用不同剂量的参考百日咳疫苗处理的样品中观察到的毒性作用程度之间呈正相关。这 13 个基因组的量化是疫苗毒性相关反应的指标。 (C) 2008 Elsevier Ltd. 保留所有权利。
Although vaccines are routinely used to prevent infectious diseases, little is known about the comprehensive influences caused by vaccines. In this study, we showed, using comprehensive gene expression analysis, that pertussis vaccine affected many genes in multiple organs of vaccine-treated animals. In particular, lung was revealed to be the most suitable target to evaluate pertussis vaccine toxicity. The 13 genes identified from the analysis of vaccine-treated lung at day I showed a clear dendrogram corresponding to pertussis vaccine toxicity. Furthermore, quantitative analysis of these genes revealed a positive correlation between their respective expression levels and the degree of toxic effects observed in samples that had been treated with various doses of reference pertussis vaccines. The quantification of this 13 gene-set is an indicator of the vaccine toxicity-related reaction. (C) 2008 Elsevier Ltd. All rights reserved.