Sequence conservation in the protein coding and intron regions of the engrailed transcription unit.
Sequence conservation in the protein coding and intron regions of the engrailed transcription unit.
复制标题
编码转录单元的蛋白质编码区和内含子区的序列保守性。
DOI：
10.1002/j.1460-2075.1986.tb04686.x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
O'Farrell,PH
中科院分区：
文献类型：
--
作者：
Kassis,JA;Poole,SJ;Wright,DK;O'Farrell,PH
Engrailed（en）是一个参与果蝇胚胎正常分割的基因。预测的en蛋白含有一个同源结构域和富含聚丙氨酸，聚谷氨酰胺，聚谷氨酸/天冬氨酸和丝氨酸的区域。我们已经采取了一种进化的方法来确定哪些区域可能是根本的重要性，通过检查D。virilis基因组序列与D.初级转录单位。序列同源性开始于长开放阅读框的第一个ATG，产生D. virilis和D. melanogaster蛋白。预测的氨基酸序列可分为保守和非保守结构域。C末端30%的蛋白质（包括同源结构域）是完全保守的。在N末端70%的蛋白质中，总体保守性为71%，但非保守性氨基酸变化成簇发生，并且存在高度保守序列的短片段。富含谷氨酸和天冬氨酸的区域是保守的，并且与myc蛋白家族成员中存在的18个氨基酸序列具有同源性。两种蛋白质大小的主要差异发生在非保守重复序列区域。在插入的转录单位的内含子中，有很长的保守区，这表明这种DNA可能具有重要的功能。
Engrailed (en) is a gene involved in proper segmentation of the Drosophila embryo. The predicted en protein contains a homeodomain and regions rich in polyalanine, polyglutamine, polyglutamate/aspartate and serine. We have taken an evolutionary approach to define which regions may be of fundamental importance by examining the D. virilis genomic sequence homologous to the D. melanogaster en primary transcription unit. Sequence homology begins at the first ATG of a long open reading frame yielding proteins of 584 and 552 amino acids for the D. virilis and D. melanogaster proteins, respectively. The predicted amino acid sequence can be divided into conserved and non‐conserved domains. The C‐terminal 30% of the protein (which includes the homeodomain) is completely conserved. In the N‐terminal 70% of the protein, the overall conservation is 71%, but non‐conservative amino acid changes occur in clusters and there are short stretches of highly conserved sequence. A region rich in glutamate and aspartate is conserved and has homology to an 18‐amino acid sequence present in members of the myc family of proteins. Major differences in the size of the two proteins occur in regions of non‐conserved repeated sequences. In the introns of the engrailed transcription units there are long stretches of conservation, suggesting this DNA may be of functional importance.
DOI：
--
发表时间：
1987
期刊：
影响因子：
--
作者：
W. Reznikoff;C. Gross;R. Burgess;Record Mt;J. Dahlberg;M. Wickens
通讯作者：
M. Wickens