Overexpression of GmFDL19 enhances tolerance to drought and salt stresses in soybean.
Overexpression of GmFDL19 enhances tolerance to drought and salt stresses in soybean.
复制标题
GmFDL19 过度表达增强大豆对干旱和盐胁迫的耐受性
DOI：
10.1371/journal.pone.0179554
复制
发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Cao D
中科院分区：
文献类型：
--
作者：
Li Y;Chen Q;Nan H;Li X;Lu S;Zhao X;Liu B;Guo C;Kong F;Cao D
碱性亮氨酸拉链（basic leucine zipper，bZIP）转录因子家族在植物的生长发育过程中起着重要作用，并对干旱、高盐等非生物胁迫有着重要的响应。我们的前期工作证实了bZIP转录因子GmFDL 19是大豆开花的启动子，并且GmFDL 19的过表达导致转基因大豆提前开花。在这里，我们报道GmFDL 19还增强大豆对干旱和盐胁迫的耐受性。GmFDL 19被确定为A组成员，其转录表达受到脱落酸（阿坝）、聚乙二醇（PEG 6000）和高盐胁迫的高度诱导。过量表达GmFDL 19可提高大豆苗期的耐旱性和耐盐性。PEG和盐处理后，转基因植株的相对株高和相对地上部干重显著高于野生型。此外，转基因大豆的发芽率和株高也显著高于野生型大豆。此外，我们还发现GmFDL 19可以减少转基因大豆中Na+离子的积累，并上调几个阿坝/胁迫响应基因的表达。我们还发现GmFDL 19过表达增加了几种抗氧化酶的活性和叶绿素含量，但降低了丙二醛含量。这些结果表明，GmFDL 19基因参与了大豆的非生物胁迫反应，在提高转基因大豆的多重胁迫抗性方面具有潜在的应用价值。
The basic leucine zipper (bZIP) family of transcription factors plays an important role in the growth and developmental process as well as responds to various abiotic stresses, such as drought and high salinity. Our previous work identified GmFDL19, a bZIP transcription factor, as a flowering promoter in soybean, and the overexpression of GmFDL19 caused early flowering in transgenic soybean plants. Here, we report that GmFDL19 also enhances tolerance to drought and salt stress in soybean. GmFDL19 was determined to be a group A member, and its transcription expression was highly induced by abscisic acid (ABA), polyethylene glycol (PEG 6000) and high salt stresses. Overexpression of GmFDL19 in soybean enhanced drought and salt tolerance at the seedling stage. The relative plant height (RPH) and relative shoot dry weight (RSDW) of transgenic plants were significantly higher than those of the WT after PEG and salt treatments. In addition, the germination rate and plant height of the transgenic soybean were also significantly higher than that of WT plants after various salt treatments. Furthermore, we also found that GmFDL19 could reduce the accumulation of Na+ ion content and up-regulate the expression of several ABA/stress-responsive genes in transgenic soybean. We also found that GmFDL19 overexpression increased the activities of several antioxidative enzyme and chlorophyll content but reduced malondialdehyde content. These results suggested that GmFDL19 is involved in soybean abiotic stress responses and has potential utilization to improve multiple stress tolerance in transgenic soybean.