Platelet-rich plasma-loaded chitosan scaffolds: Preparation and growth factor release kinetics
Platelet-rich plasma-loaded chitosan scaffolds: Preparation and growth factor release kinetics
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DOI：
10.1002/jbm.b.32806
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发表时间：
2013-01-01
影响因子：
3.4
通讯作者：
Nohutcu, Rahime M.
中科院分区：
文献类型：
--
作者：
Kutlu, Burak;Aydin, Rahime Seda Tigli;Nohutcu, Rahime M.
本研究的目的是比较不同富血小板血浆（PRP）制备方法对血小板活性的影响，并研究用于组织工程应用的负载 PRP 的壳聚糖支架的生长因子（GF）释放动力学。流式细胞术分析表明，通过所研究的标记物，用于 PRP 制备的离心过程不会对血小板活化水平产生显着影响。使用两种不同的方法来制备负载 PRP 的壳聚糖支架：（i）在冷冻干燥前将 PRP 添加到壳聚糖凝胶中以制备支架（称为 GEL）；（ii）将 PRP 嵌入到冻干壳聚糖支架中以制备支架（称为 SPONGE）。此外，将未活化的PRP和用I型胶原活化的PRP用作对照组。扫描电子显微镜图像表明，在 GEL 组中，支架的多孔、3D 和互连结构没有恶化。通过酶联免疫吸附测定测定血小板衍生的 GF-BB、转化 GF-β 1 和胰岛素样 GF-1 的 GF 释放动力学。 GEL 组实现了 GF 的持续释放，而 SPONGE 组的所有 GF 均观察到急剧的爆发释放。此外，GEL 组中血小板源性 GF-BB、类胰岛素 GF-1 和转化 GF-β 1 的释放时间延长至 20 天，并且从 GEL 和 SPONGE 支架中释放的所有 GF 的生物活性均得以保留。这项研究表明，称为 GEL 的壳聚糖支架可以通过提供 GF 的持续释放而成为 PRP 应用的合适载体。 (c) 2012 年 Wiley periodicals, Inc. J Biomed Mater Res B 部分：Appl Biomater 101B：2835，2013。
The aim of this study is to compare the effects of different platelet-rich plasma (PRP) preparation methods on platelet activity and to investigate the growth factor (GF) release kinetics from PRP-loaded chitosan scaffolds for tissue engineering applications. Flow cytometry analysis showed that centrifugation processes used for PRP preparation did not cause significant effect on platelet activation levels by means of markers investigated. Two different methods were used to prepare PRP-loaded chitosan scaffolds: (i) PRP was added to chitosan gel before freeze-drying to prepare scaffolds called as GEL and (ii) PRP was embedded to freeze-dried chitosan scaffolds to prepare scaffolds called as SPONGE. In addition, nonactivated PRP and PRP activated with type-I collagen were used as control groups. Scanning electron microscopy images demonstrated that, in GEL group, there is no deterioration on the scaffolds porous, 3D, and interconnected structure. GF release kinetics was determined by enzyme-linked immunosorbent assay for platelet-derived GF-BB, transforming GF-beta 1, and insulin-like GF-1. A sustained release of GFs was achieved in GEL group while a sharp burst release was observed for all the GFs from the SPONGE groups. Moreover, platelet-derived GF-BB, insulin-like GF-1, and transforming GF-beta 1 releases were prolonged to 20 days in GEL groups, and the biological activities of all GFs released from GEL and SPONGE scaffolds were preserved. This study demonstrated that chitosan scaffold that was called GEL could be an appropriate carrier for PRP applications by providing sustained release of GFs. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 101B: 2835, 2013.