Production of three-dimensional silk fibroin nanofiber non-woven fabric by wet electrospinning
Production of three-dimensional silk fibroin nanofiber non-woven fabric by wet electrospinning
复制标题
湿法静电纺丝生产三维丝素纳米纤维非织造布
DOI：
10.11417/silk.25.49
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Tamada Y.
中科院分区：
文献类型：
--
作者：
Kisimoto Y;Kobashi T;Morikawa H;Tamada Y.
采用湿式静电纺丝技术，以丝素水纺丝液为捕收剂，制备了三维丝素纳米纤维无纺布。液浴采用pH 3.8的柠檬酸缓冲液加t-丁醇（t-BuOH）。t-BuOH浓度对WES非织造布的表观孔径和孔隙率有影响。在t-BuOH浓度为30%时形成最大孔径。纺态WES非织造布结构呈结晶形态。与传统干静电纺丝（DES）非织造布相比，WES非织造布上的细胞粘附力较低，但细胞增殖率与DES非织造布无显著差异。细胞在WES无纺布的表面和内部空间都有粘附和增殖，而细胞只在高纤维密度的DES无纺布表面有粘附。WES无纺布有望用作细胞支架，模拟组织中的细胞外基质。(*：邮件地址：ytamada@ shinshu-u。ac. jp)
Three-dimensional (3D) silk fibroin (SF) nanofiber non-woven fabric was fabricated by wet electrospinning (WES) using SF aqueous spinning solution and a liquid bath as a collector. Citric acid buffered solution at pH 3.8 supplemented with t-butyl alcohol (t-BuOH) was used as the liquid bath. The t-BuOH concentration influenced the apparent pore size and porosity of the WES nonwoven fabric. The maximum pore size was formed at 30% of t-BuOH. The as-spun WES nonwoven fabric structure had a crystallized form. Cell adhesion on the WES non-woven fabric was lower than that produced on the non-woven fabric by traditional dry electrospinning (DES), but no difference was observed in cell proliferation rates between WES and DES non-woven fabrics. Cells adhered and proliferated on both the surface and the inner spaces of WES non-woven fabric, although cells adhered only on the surface of DES non-woven fabric consisting of high fiber density. The WES non-woven fabric is anticipated for use as a cell scaffold mimicking an extracellular matrix in tissue.(*: To whom correspondence should be addressed, E mail: ytamada@ shinshu-u. ac. jp)