Effects of Minocycline on Hind-Limb Motor Function and Gray and White Matter Injury After Spinal Cord Ischemia in Rats
Effects of Minocycline on Hind-Limb Motor Function and Gray and White Matter Injury After Spinal Cord Ischemia in Rats
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DOI：
10.1097/brs.0b013e3181ffda29
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发表时间：
2011-11
期刊：
影响因子：
3
通讯作者：
Masafumi Takeda;M. Kawaguchi;Tomoyuki Kumatoriya;T. Horiuchi;Keisuke Watanabe;S. Inoue;N. Konishi;H. Furuya
中科院分区：
文献类型：
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作者：
Masafumi Takeda;M. Kawaguchi;Tomoyuki Kumatoriya;T. Horiuchi;Keisuke Watanabe;S. Inoue;N. Konishi;H. Furuya
研究设计.一项前瞻性、随机化实验室研究。Objective.研究米诺环素是否能减轻脊髓缺血后后肢运动功能障碍和灰质及白色物质损伤。背景数据总结。米诺环素是一种半合成的四环素类抗生素，已被证明在局灶性和全脑缺血模型中具有神经保护作用。然而，还没有关于米诺环素在脊髓缺血模型中的作用的数据。方法.将36只大鼠随机分为对照组（C组，n = 11）、二甲胺四环素组（M组，n = 13）和假手术组（n = 12）。在脊髓缺血或假手术前10分钟12小时开始腹膜内给予米诺环素或生理盐水，持续3天。采用主动脉内球囊导管和放血法造成脊髓缺血。再灌注后72 h，采用Basso，Beattie，Bresnahan（BBB）评分法（0 =截瘫，21 =正常）评定后肢运动功能。对于组织学评估，分别使用正常神经元的数量和白色物质中空泡化的程度来评估灰质和白色物质损伤。还使用Iba-1免疫组织化学评价了活化的小胶质细胞。结果M组的BBB评分和正常神经元数目均显著高于C组。与C组相比，M组的白色物质中空泡的面积百分比和Iba-1阳性细胞的数量显著降低。结论结果表明，米诺环素给药改善大鼠后肢运动功能，减轻脊髓缺血后灰质和白色质损伤及小胶质细胞活化。
Study Design. A prospective, randomized laboratory investigation. Objective. To investigate whether administration of minocycline attenuates hind-limb motor dysfunction and gray and white matter injury after spinal cord ischemia. Summary of Background Data. Minocycline, a semisynthetic tetracycline antibiotic, has been shown to have neuroprotective effects in models of focal and global cerebral ischemia. However, there have been no data available regarding the effects of minocycline in a model of spinal cord ischemia. Methods. Thirty-six rats were randomly allocated to one of three groups; control (C) group (n = 11), minocycline (M) group (n = 13), or sham group (n = 12). Minocycline or saline was intraperitoneally administered for 3 days beginning at 12 hours before 10 minutes of spinal cord ischemia or sham operation. Spinal cord ischemia was induced with intraaortic balloon catheter and blood withdrawal. Seventy-two hours after reperfusion, hind-limb motor functions were assessed using Basso, Beattie, Bresnahan (BBB) Scale (0 = paraplegia, 21 = normal). For histologic assessments, the gray and white matter injury was evaluated using the number of normal neurons and the extents of vacuolations in the white matter, respectively. Activated microglia was also evaluated using Iba-1 immunohistochemistry. Results. BBB scores and the numbers of normal neurons in the M group were significantly higher than those in the C group. The percentage areas of vacuolations in the white matter and the number of Iba-1 positive cells were significantly lower in the M group compared with those in the C group. Conclusion. The results indicated that minocycline administration improved hind-limb motor function and attenuated gray and white matter injury and microglial activation after spinal cord ischemia in rats.