Crx, a novel otx-like homeobox gene, shows photoreceptor-specific expression and regulates photoreceptor differentiation
Crx, a novel otx-like homeobox gene, shows photoreceptor-specific expression and regulates photoreceptor differentiation
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DOI：
10.1016/s0092-8674(00)80439-0
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发表时间：
1997-11-14
期刊：
影响因子：
64.5
通讯作者：
Cepko, CL
中科院分区：
文献类型：
--
作者：
Furukawa, T;Morrow, EM;Cepko, CL
我们已经从小鼠视网膜中分离出一种新的类似于otx的同源盒基因Crx。Crx的表达仅限于发育和成熟的感光细胞。CRX结合并反激活了TAATCC/A序列，该序列位于几个光受体特异性基因的上游，包括许多物种的视蛋白基因。使用逆转录病毒载体过表达Crx增加了含有纯杆状光感受器的克隆的频率，减少了含有无分泌中间神经元和Muller胶质细胞的克隆的频率。此外，假设表达CRX显性阴性形式的光感受器细胞未能形成适当的光感受器外段和终末。Crx是一种新型的光感受器特异性转录因子，在光感受器细胞的分化中起着至关重要的作用。
We have isolated a novel otx-like homeobox gene, Crx, from the mouse retina. Crx expression is restricted to developing and mature photoreceptor cells. CRX bound and transactivated the sequence TAATCC/A, which is found upstream of several photoreceptor-specific genes, including the opsin genes from many species. Overexpression of Crx using a retroviral vector increased the frequency of clones containing exclusively rod photoreceptors and reduced the frequency of clones containing amacrine interneurons and Muller glial cells. In addition, presumptive photoreceptor cells expressing a dominant-negative form of CRX failed to form proper photoreceptor outer segments and terminals. Crx is a novel photoreceptor-specific transcription factor and plays a crucial role in the differentiation of photoreceptor cells.