Tandem mass tag proteomic and untargeted metabolomic profiling reveals altered serum and CSF biochemical datasets in iron deficient monkeys.
Tandem mass tag proteomic and untargeted metabolomic profiling reveals altered serum and CSF biochemical datasets in iron deficient monkeys.
复制标题
DOI：
10.1016/j.dib.2022.108591
复制
发表时间：
2022-12
期刊：
影响因子：
1.2
通讯作者：
中科院分区：
文献类型：
--
作者：
早期生活缺铁性贫血（IDA）的影响延伸到血液之外，包括对包括大脑在内的许多组织的短期和长期不利影响。在IDA之前，缺铁（ID）可以引起类似的组织效应，但缺乏铁依赖性脑健康的敏感生物标志物。为了确定id诱导的代谢功能障碍的血清和脑脊液生物标志物，我们对婴儿IDA非人灵长类动物模型4个月和6个月时的血清和脑脊液进行了蛋白质组学和代谢组学分析。LC/MS/MS分析共鉴定出血清中227种代谢物和205种蛋白质。在脑脊液中，我们测量了210种代谢物和1560种蛋白质。数据由Q-Exactive （Thermo Scientific, Waltham， MA）通过Progenesis QI进行处理，并与专有的小分子数据库和Metlin进行精确的质量和保留时间比较，或者直接从Fusion Orbitrap （Thermo Scientific, Waltham， MA）通过Proteome Discoverer 2.4.0.305 （Thermo Scientific, Waltham， MA）中的Sequest导入原始文件，并通过最新的恒河猴HMDB数据库进行肽匹配。代谢产物和蛋白质标识符、p值和q值用于独创性路径分析（Ingenuity Pathways analysis， IPA）的分子途径分析。我们应用多路距离加权辨别（DWD）来确定在4个月大（贫血前期）和6个月大（贫血）时区分ID和IS的特征（蛋白质或代谢物）的加权和。
The effects of early-life iron deficiency anemia (IDA) extend past the blood and include both short- and long-term adverse effects on many tissues including the brain. Prior to IDA, iron deficiency (ID) can cause similar tissue effects, but a sensitive biomarker of iron-dependent brain health is lacking. To determine serum and CSF biomarkers of ID-induced metabolic dysfunction we performed proteomic and metabolomic analysis of serum and CSF at 4- and 6- months from a nonhuman primate model of infantile IDA. LC/MS/MS analyses identified a total of 227 metabolites and 205 proteins in serum. In CSF, we measured 210 metabolites and 1,560 proteins. Data were either processed from a Q-Exactive (Thermo Scientific, Waltham, MA) through Progenesis QI with accurate mass and retention time comparisons to a proprietary small molecule database and Metlin or with raw files imported directly from a Fusion Orbitrap (Thermo Scientific, Waltham, MA) through Sequest in Proteome Discoverer 2.4.0.305 (Thermo Scientific, Waltham, MA) with peptide matches through the latest Rhesus Macaque HMDB database. Metabolite and protein identifiers, p-values, and q-values were utilized for molecular pathway analysis with Ingenuity Pathways Analysis (IPA). We applied multiway distance weighted discrimination (DWD) to identify a weighted sum of the features (proteins or metabolites) that distinguish ID from IS at 4-months (pre-anemic period) and 6-months of age (anemic).