A preferential binding site for insulin-like growth factor II in human and rat placental membranes.
A preferential binding site for insulin-like growth factor II in human and rat placental membranes.
复制标题
人和大鼠胎盘膜中胰岛素样生长因子 II 的优先结合位点。
DOI：
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发表时间：
1981
影响因子：
5.8
通讯作者：
B. Trivedi
中科院分区：
文献类型：
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作者：
W. Daughaday;I. Mariz;B. Trivedi
我们比较了结合胰岛素样生长因子I和II（IGF-I和IGF-II）的部分纯化的人和大鼠胎盘膜。当[125 I]IGF-I与人胎盘膜结合时，IGF-I比IGF-II更有效地置换示踪剂。对IGF-I结合的Scatchard分析提供了高亲和力、低容量结合系统和第二低亲和力结合系统的证据。当[125 I]IGF-II与人胎盘膜结合时，它比IGF-I更容易被IGF-II置换。结合IGF-II的Scatchard分析表明，结合的高亲和力组分减少，IGF-II的大部分结合是由于较低的亲和力和较高的容量系统。与人胎盘相比，大鼠胎盘膜结合[125 I]IGF-I相对较弱，IGF-II在取代该标记方面比IGF-I和大鼠生长调节素更有效。与此相反，[125 I]IGF-II与大鼠胎盘膜的结合大于[125 I]IG。
We have compared the binding of insulin-like growth factors I and II (IGF-I and IGF-II) by partially purified human and rat placental membranes. When [125I]IGF-I wasbound by human placental membranes, IGF-I was more effective than IGF-II in displacing the tracer. Scatchard analysis of the binding of IGF-I provided evidence of a high affinity, low capacity binding system and a second lower affinity binding system. When [125I]IGF-II was bound by human placental membranes, it was displaced much more readily by IGF-II than by IGF-I. Scatchard analysis of the binding of IGF-II indicated that the high affinity component of binding was reduced, and most of the binding of IGF-II was due to the lower affinity and higher capacity system. In comparison to human placenta, rat placental membranes bound [125I]IGF-I relatively weakly, the IGF-II was more effective than IGF-I and rat somatomedin in displacing this label. In contrast, the binding of [125I]IGF-II by rat placental membranes was greater than that of [125I]IG...