Cloning and characterisation of a pepper aquaporin, CaAQP, which reduces chilling stress in transgenic tobacco plants
Cloning and characterisation of a pepper aquaporin, CaAQP, which reduces chilling stress in transgenic tobacco plants
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DOI：
10.1007/s11240-014-0495-3
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发表时间：
2014-04
期刊：
影响因子：
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通讯作者：
Yanxu Yin;Wei-Li Guo;Yinglei Zhang;J-J Ji-J;Huaijuan Xiao;Fei Yan;Yan-yan Zhao;Wen-Chao Zhu;Rugang Chen;W. Chai;Z. Gong
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文献类型：
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作者：
Yanxu Yin;Wei-Li Guo;Yinglei Zhang;J-J Ji-J;Huaijuan Xiao;Fei Yan;Yan-yan Zhao;Wen-Chao Zhu;Rugang Chen;W. Chai;Z. Gong
被称为水通道蛋白的细胞膜蛋白是主要内在蛋白（MIP）的成员，其控制水分子跨细胞膜的特异性运输。从辣椒（Capsicum annuumL.）低温处理的幼苗叶片中分离到一个具有MIP亚家族液泡膜内在蛋白结构特征的辣椒水通道蛋白基因（CaAQP）。CV. P70。分析表明在辣椒的幼子、绿色果实和花蕾中表达水平高，而在茎、叶和根中表达水平低。HgCl 2、低温、脱落酸、氟啶酮和渗透胁迫均能快速诱导表达。辣椒幼苗在低温下用脱落酸预处理的反应表明，低温上调CaAQP，这可能是参与阿坝信号。结果表明，CaAQP基因的过表达能够降低转基因植株的低温胁迫，可能是通过增加胁迫下的气孔开度，增加恢复期的膜损伤率，从而影响胞间CO2浓度，降低气孔导度和蒸腾速率。VIGS的CaAQPin辣椒植物造成显着的生长迟缓。这些结果表明CaAQP在植物对非生物胁迫的响应中起着重要作用。
Ubiquitous cell membrane proteins called aquaporins are members of major intrinsic proteins (MIPs), which control the specific transport of water molecules across cell membranes. A pepper aquaporin gene (CaAQP), which exhibits the structural features of tonoplast intrinsic proteins of the MIP subfamily, was isolated from the leaves of chilling-treated seedlings of pepper (Capsicum annuumL.) cv. P70. Assays indicated high levels of expression in young seeds, green fruits and flower buds and low levels of expression in the stems, leaves and roots of pepper. The expression patterns were strongly and rapidly induced by HgCl2, low temperature, abscisic acid, fluridone and osmotic stresses. The responsiveness of pepper seedlings pretreated with abscisic acid at low temperatures demonstrated up-regulation ofCaAQPby chilling, which is potentially involved in ABA signalling. Our results indicated that overexpression ofCaAQPdecreased chilling stress in transgenic plants, likely by increasing the stomatal aperture under stress, increasing the rate of membrane damage during the recovery stage, thereby affecting the intercellular CO2concentration with lower stomatal conductance and transpiration rates. VIGS ofCaAQPin pepper plants caused significant growth retardation. These results suggested thatCaAQPplayed a crucial role in the plant response to abiotic stresses.