Characterization of a novel polymeric scaffold for potential application in tendon/ligament tissue engineering
Characterization of a novel polymeric scaffold for potential application in tendon/ligament tissue engineering
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DOI：
10.1089/ten.2006.12.91
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发表时间：
2006-01-01
影响因子：
--
通讯作者：
Toh, SL
中科院分区：
文献类型：
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作者：
Sahoo, S;Ouyang, H;Toh, SL
与编织织物不同，针织支架已被证明有利于胶原结缔组织基质的沉积，这对于肌腱/韧带重建至关重要。但是这种支架的细胞接种通常需要凝胶系统，这在动态情况下是不稳定的，特别是在膝关节中。本研究开发了一种新型的，可生物降解的纳米微纤维聚合物支架，通过静电纺丝PLGA纳米纤维到针织PLGA支架，以提供一个大的仿生表面的细胞附着。将猪骨髓基质细胞通过移液细胞悬液接种到新型支架上（第I组）或通过固定在纤维蛋白凝胶中接种到编织的PLGA支架上（第II组）。研究了36小时时的细胞附着、1周时的细胞增殖和细胞外基质合成以及2周以上的机械性能。组I中细胞附着相当，细胞增殖更快。此外，细胞功能在组I中表现得更活跃，如通过I型胶原蛋白、核心蛋白聚糖和双糖链蛋白聚糖基因的较高表达所证明的。因此，这种新型支架，促进细胞接种和促进细胞增殖，功能和分化，可以应用于组织工程肌腱/韧带的承诺。
Unlike braided fabrics, knitted scaffolds have been proven to favor deposition of collagenous connective tissue matrix, which is crucial for tendon/ligament reconstruction. But cell seeding of such scaffolds often requires a gel system, which is unstable in a dynamic situation, especially in the knee joint. This study developed a novel, biodegradable nano-microfibrous polymer scaffold by electrospinning PLGA nanofibers onto a knitted PLGA scaffold in order to provide a large biomimetic surface for cell attachment. Porcine bone marrow stromal cells were seeded onto either the novel scaffolds by pipetting a cell suspension (Group I) or the knitted PLGA scaffolds by immobilizing in fibrin gel (Group II). Cell attachment at 36 hours, cell proliferation and extracellular matrix synthesis at 1 week, and mechanical properties over 2 weeks were investigated. Cell attachment was comparable and cell proliferation was faster in Group I. Moreover, cellular function was more actively exhibited in Group I, as evident by the higher expression of collagen I, decorin, and biglycan genes. Thus, this novel scaffold, facilitating cell seeding and promoting cell proliferation, function, and differentiation, could be applied with promise in tissue engineering of tendon/ligament.