Electrophysiological changes correlated with temperature increases induced by high-intensity focused ultrasound ablation.
Electrophysiological changes correlated with temperature increases induced by high-intensity focused ultrasound ablation.
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DOI：
10.1016/j.ultrasmedbio.2014.09.009
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发表时间：
2015-02
影响因子：
2.9
通讯作者：
Deng, Cheri X.
中科院分区：
文献类型：
--
作者：
Wu, Ziqi;Kumon, Ronald E.;Laughner, Jacob I.;Efimov, Igor R.;Deng, Cheri X.
关键词：
为了更好地了解高强度聚焦超声(HIFU)消融治疗心律失常的详细机制，我们利用Langendorff灌流的兔心，研究了HIFU应用期间细胞电生理(EP)变化与温度升高和热剂量(累积当量分钟数[CEM43])之间的关系。使用压敏染料di-4-ANEPPS，利用同步光学标测和红外成像技术测量了HIFU过程中的EP和温度。动作电位幅度(APA)和动作电位复极50%时程(APD50)均随温度升高而降低，且APD50的温度敏感性高于APA。当HIFU诱导的温度高于5 2.3±1.4℃和log10(CEM4 3)高于2.16±0.5 1(n=5)时，EP和组织的改变是不可逆的(n=5)，而当温度低于5 0.1±0.8℃和log10(CEM4 3)低于−0.9±0.3(n=9)时，EP和组织的改变是可逆的。EP和温度/热剂量的变化与HIFU诱导的周围有过渡区的组织坏死存在空间相关性。
To gain better understanding of the detailed mechanisms of high-intensity focused ultrasound (HIFU) ablation for cardiac arrhythmias, we investigated how the cellular electrophysiological (EP) changes were correlated with temperature increases and thermal dose (cumulative equivalent minutes [CEM43]) during HIFU application using Langendorff-perfused rabbit hearts. Employing voltage-sensitive dye di-4-ANEPPS, we measured the EP and temperature during HIFU using simultaneous optical mapping and infrared imaging. Both action potential amplitude (APA) and AP duration at 50% repolarization (APD50) decreased with temperature increases, and APD50 was more thermally sensitive than APA. EP and tissue changes were irreversible when HIFU-induced temperature increased above 52.3 ± 1.4 °C and log10(CEM43) above 2.16 ± 0.51 (n = 5), but were reversible when temperature was below 50.1 ± 0.8 °C and log10(CEM43) below −0.9 ± 0.3 (n = 9). EP and temperature/thermal dose changes were spatially correlated with HIFU induced tissue necrosis surrounded by a transition zone.
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影响因子：
6.1
作者：
Metzner, Andreas;Chun, K. R. Julian;Schmidt, Boris
通讯作者：
Schmidt, Boris
DOI：
10.1161/circep.111.967216
发表时间：
2012-04
期刊：
Circulation. Arrhythmia and electrophysiology
影响因子：
--
作者：
Laughner JI;Sulkin MS;Wu Z;Deng CX;Efimov IR
通讯作者：
Efimov IR
影响因子：
37.8
作者：
Nanthakumar, K;Plumb, VJ;Kay, GN
通讯作者：
Kay, GN
影响因子：
3.3
作者：
Hsiao, Yi-Sing;Kumon, Ronald E.;Deng, Cheri X.
通讯作者：
Deng, Cheri X.
影响因子：
4.8
作者：
KANNEL, WB;CUPPLES, LA;DAGOSTINO, RB
通讯作者：
DAGOSTINO, RB