DELINEATION OF BORRELIA-BURGDORFERI SENSU-STRICTO, BORRELIA-GARINII SP-NOV, AND GROUP VS461 ASSOCIATED WITH LYME BORRELIOSIS
DELINEATION OF BORRELIA-BURGDORFERI SENSU-STRICTO, BORRELIA-GARINII SP-NOV, AND GROUP VS461 ASSOCIATED WITH LYME BORRELIOSIS
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DOI：
10.1099/00207713-42-3-378
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发表时间：
1992-07-01
期刊：
影响因子：
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通讯作者：
GRIMONT, PAD
中科院分区：
文献类型：
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作者：
BARANTON, G;POSTIC, D;GRIMONT, PAD
我们研究了48株与莱姆病相关或从蜱虫中分离的疏螺旋体分离株，并使用S1核酸酶方法鉴定了三个DNA相关性组。这三个DNA组（基因种）与特定的rRNA基因限制性图谱、蛋白电泳图谱和与鼠单克隆抗体的反应性图谱相关。基因种I对应于狭义伯氏疏螺旋体，因为它含有该种的模式菌株（菌株ATCC 35210）;该基因种包括来自欧洲和美国的28株分离株。基因种II被命名为Borrelia garinii sp. nov.，包括来自欧洲和日本的13个分离株。基因种III（组VS 461）包括来自欧洲和日本的7株分离株。
We studied 48 Borrelia isolates that were associated with Lyme borreliosis or were isolated from ticks and identified three DNA relatedness groups by using the S1 nuclease method. The three DNA groups (genospecies) were associated with specific rRNA gene restriction patterns, protein electrophoresis patterns, and patterns of reactivity with murine monoclonal antibodies. Genospecies I corresponded to Borrelia burgdorferi sensu stricto since it contained the type strain of this species (strain ATCC 35210); this genospecies included 28 isolates from Europe and the United States. Genospecies II was named Borrelia garinii sp. nov. and included 13 isolates from Europe and Japan. Genospecies III (group VS461) included seven isolates from Europe and Japan.