Antagonizing effect of ISA on lipid peroxidation of vein endothelial cells
Antagonizing effect of ISA on lipid peroxidation of vein endothelial cells
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发表时间：
2007
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通讯作者：
Sun Jian-ning
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作者：
Sun Jian-ning
目的研究异亚丙基莽草酸（伊萨）对过氧化氢（H2 O2）诱导的人脐静脉内皮细胞（HUVEC）脂质过氧化损伤的影响。方法采用硫代巴比妥酸（TBA）法测定HUVEC中丙二醛（MDA）含量，黄嘌呤氧化酶法测定超氧化物歧化酶（SOD）活性，可见光谱法测定过氧化氢酶（CAT）活性。结果伊萨（10-5，10-4 mol/L）可明显减轻H2 O2（200 μmol/L）诱导的HUVEC脂质过氧化反应，降低MDA含量，保护CAT和SOD活性。伊萨作用HUVEC 12 h后，SOD和CAT活性均明显升高。伊萨（10-5~10-3 mol/L）对超氧阴离子有明显的清除作用，且具有一定的质效关系。结论伊萨对体外培养的血管内皮细胞脂质过氧化有拮抗作用，但对SOD和CAT活性无直接影响。
Objective To study the influence of 3, 4-oxo-isopropylidene-shikimic acid (ISA) on lipid peroxidation of human umbilical vein endothelial cells (HUVEC) induced by hydrogen peroxide (H2O2) in vitro. Methods The concentration of malonaldehyde (MDA) in HUVEC was detected by thiobarbituric acid (TBA) method, the activities of superoxide dismutase (SOD) was detected by xanthine oxidase system and catalase (CAT) was detected by visible spectrum assay. The clearance (C) of superoxide anion and OH radicals by ISA were tested by free radical prehensile experiment in vitro.Results The lipid peroxidation induced by H2O2 (200 μmol/L for 6 hours) was alleviated obviously in HUVEC cultured with ISA (10-5, 10-4 mol/L) for 6 hours, MDA was decreased and the activities of CAT and SOD were protected. The activities of SOD and CAT were improved after HUVEC incubation with ISA for 12 hours. ISA (10-5~10-3 mol/L) had an obvious clearing effect on superoxide anion, showed a relationship between quality and effectiveness. ISA (10-3 mol/L) could also clear OH radicals.Conclusion ISA has an antagonizing effect on lipid peroxidation of vein endothelial cells in vitro, but no direct influence on the activities of SOD and CAT.