THE EFFECT OF LIPIDS AND SERUM ALBUMIN ON BACTERIAL GROWTH.
THE EFFECT OF LIPIDS AND SERUM ALBUMIN ON BACTERIAL GROWTH.
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DOI：
10.1084/jem.85.1.9
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发表时间：
1947-01-01
期刊：
影响因子：
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通讯作者：
Dubos RJ
中科院分区：
文献类型：
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作者：
Dubos RJ
已发现长链脂肪酸对结核杆菌和某些未鉴定的微球菌培养物的生长具有抑制和刺激作用。通过（a）酯化（即使所得产物为水溶性酯）和（B）向培养基中添加结晶血清白蛋白，脂肪酸的毒性大大降低或消除;其他测试蛋白在这方面无活性。当某些长链脂肪酸以其水溶性酯的形式或与足量的血清白蛋白混合时，所研究的微生物的显著生长刺激被获得。在含有葡萄糖作为唯一碳源的矿物培养基中添加油酸、亚油酸、亚麻酸或花生四烯酸（0.0001%至0.001%），导致微球菌的大量生长;在这种微生物种属的情况下，测试的其他物质都不能替代这些不饱和脂肪酸。在培养基中加入0.001%~ 0.01%的各种脂肪酸（饱和或不饱和），即使在不含葡萄糖或任何其他容易获得的碳化合物的情况下，也能促进结核杆菌的生长。这些结果表明，长链脂肪酸可以通过不同的代谢途径影响不同微生物物种的生长，并且为了研究这些代谢和生长反应的机制，必须在它们不能表现出其毒性的条件下使用脂肪酸。
Long chain fatty acids have been found to exhibit both inhibitory and stimulatory effects on the growth of tubercle bacilli and of a certain unidentified micrococcus culture. The toxicity of the fatty acids was much reduced or abolished by (a) esterification, even when the resulting product was a water-soluble ester, and (b) addition of crystalline serum albumin to the culture medium; other proteins tested were inactive in this respect. Marked growth stimulation of the microorganisms studied was obtained when certain long chain fatty acids were added to the culture medium in the form of their water-soluble esters, or in admixture with adequate amounts of serum albumin. Abundant growth of the micrococcus resulted from the addition of oleic, linoleic, linolenic, or arachidonic acid (0.0001 to 0.001 per cent) to a mineral medium containing glucose as sole source of carbon; in the case of this microbial species, none of the other substances tested could substitute for these unsaturated fatty acids. Enhancement of growth of tubercle bacilli was obtained by adding to the medium 0.001 to 0.01 per cent of a variety of fatty acids (saturated or unsaturated) even in the absence of glucose or of any other readily available carbon compound. These results suggest that long chain fatty acids can affect the growth of different microbial species through different metabolic channels and that, in order to study the mechanism of these metabolic and growth reactions, it is essential to use the fatty acids under conditions where they cannot manifest their toxic properties.