Cloning of a human heptahelical receptor closely related to the P2Y5 receptor.
Cloning of a human heptahelical receptor closely related to the P2Y5 receptor.
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与 P2Y5 受体密切相关的人类七螺旋受体的克隆。
DOI：
10.1006/bbrc.1997.6895
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发表时间：
1997
影响因子：
3.1
通讯作者：
D. Communi
中科院分区：
文献类型：
--
作者：
R. Janssens;J. Boeynaems;M. Godart;D. Communi
从活化的鸡T细胞克隆的6 H1受体最初被认为是孤儿G偶联受体，但后来基于显著的氨基酸同一性被包括在嘌呤和嘧啶核苷酸的P2 Y受体家族中，并被重命名为P2 Y 5。表达的序列标签数据库的分析揭示了一个相关的序列表现出63%的氨基酸同一性与该受体的存在。从这个部分序列开始，我们已经分离出一个完整的克隆，并确定了一个1113个碱基对的开放阅读框架编码一个新的G-偶联受体，我们称之为P2 Y 5样。该序列与鸡P2 Y 5序列具有61%的同一性，与其他P2 Y亚型具有30-33%的同一性。将编码这种P2 Y 5样受体的构建体转染到COS-7、1321 N1和CHO-K1细胞中，并通过北方印迹记录表达。没有40个核苷酸和核苷测试是能够引起任何四个功能测定的反应：磷酸肌醇形成，刺激或抑制cAMP的形成，和细胞外酸化用微生理计测量。这些结果表明，P2 Y 5样受体的天然配体是一种罕见的核苷酸，或者尽管它与P2 Y家族结构相似，但它不是核苷酸受体。
The 6H1 receptor cloned from activated chicken T cells was initially considered an orphan G-coupled receptor, but was later included in the P2Y family of receptors for purine and pyrimidine nucleotides on the basis of a significant amino acid identity and was renamed P2Y5. Analysis of the expressed sequence tag database revealed the presence of a related sequence exhibiting 63% amino acid identity with this receptor. Starting from this partial sequence, we have isolated a complete clone and identified a 1113 base pair open reading frame encoding a new G-coupled receptor that we have called P2Y5-like. This sequence exhibits 61% identity with the chicken P2Y5 sequence and 30-33% with other P2Y subtypes. A construct encoding this P2Y5-like receptor was transfected into COS-7, 1321N1, and CHO-K1 cells, and expression was documented by Northern blotting. None of the 40 nucleotides and nucleosides tested was able to elicit a response in any of four functional assays: inositol phosphate formation, stimulation or inhibition of cAMP formation, and extracellular acidification measured with a microphysiometer. These results suggest either that the natural ligand of the P2Y5-like receptor is an uncommon nucleotide or alternatively that despite its structural similarity to the P2Y family it is not a nucleotide receptor.
鉴定活化 T 细胞中诱导的 G 蛋白偶联受体。
DOI：
--
发表时间：
1993
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Kaplan,MH;Smith,DI;Sundick,RS
通讯作者：
Sundick,RS