Liquid Metal Microscale Deposition enabled High Resolution and Density Epidermal Microheater for Localized Ectopic Expression in Drosophila
Liquid Metal Microscale Deposition enabled High Resolution and Density Epidermal Microheater for Localized Ectopic Expression in Drosophila
复制标题
液态金属微尺度沉积实现了高分辨率和密度表皮微加热器的果蝇局部异位表达
DOI：
10.1002/admt.202100903
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发表时间：
2021-10
影响因子：
6.8
通讯作者：
Wu Zhigang
中科院分区：
文献类型：
--
作者：
Wang Bei;Gao Junjun;Jiang Jiajun;Hu Zenghui;Hjort Klas;Guo Zheng;Wu Zhigang
果蝇肠道内分泌细胞的定位异位表达为研究人类肠道疾病，如炎症性肠病提供了一个方便的模型。由于其非侵入性的特点，热激活异位表达是一个很好的选择，但它需要对微小而脆弱的活蝇腹部(≈1×1mm2)进行局部加热，而不是对全身进行整体加热。这项工作提出了一种薄型、高分辨率和高密度的液态金属(LM)微加热器，具有气体渗透性和足够的柔韧性，可以为苍蝇微小的腹部提供均匀的局部加热。开发了一种通过引入微米/亚微米颗粒来制备微米级薄(5微米)、光滑(Sa=0.8µm)、透气的微加热器用的LM薄膜的沉积技术。为了解决微米/亚微米颗粒的特殊壳层导致的低电导率问题，提出了碰撞诱导的Lm颗粒合并和烧结的方法。由于颗粒的细小，沉积可以在波状或粗糙的表面上共形打印。有了荫罩，这种微尺度的沉积可以同时实现高产量和高分辨率、高密度和高精度的薄LM图案的重现。此外，还证明了表皮贴片可以柔顺地附着在皱纹皮肤和指纹上。最后，通过将这种微型加热器应用于苍蝇腹部，成功地展示了肠道内分泌细胞的局部异位表达。
Localized ectopic expression of enteroendocrine cells in fruit flies provides a convenient model for studying human intestinal diseases, e.g., inflammatory bowel disease, that need urgent attention. Due to its noninvasive feature, thermal activating ectopic expression is an excellent option, but it demands local heating of the tiny and delicate live flies’ abdomen (≈1 × 1 mm2) without blocking their breath, instead of global heating of the whole body. This work proposes a thin, high‐resolution, and density liquid metal (LM) microheater with gas permeability and sufficient compliance to provide homogenous local heating to the fly's tiny abdomen. A depositing technique is developed that can make microscale thin (5 µm), smooth (Sa = 0.8 µm), gas permeable LM film for such microheater by introducing LM micrometer/sub‐micrometer particles. Collision induced LM particle merging and hence sintering is proposed to solve low conductivity due to their particular shells of micrometer/sub‐micrometer particles. The deposition can conformally print on the wavy or rough surface due to the fine particle size. With a shadow mask, this microscale deposition can enable high yield and reproducible thin LM patterning with high resolution, density, and precision simultaneously. Furthermore, it is demonstrated that epidermal patches can compliantly attach onto the wrinkle skin and fingerprint. Finally, by applying such a microheater on the fly abdomen, localized ectopic expression of enteroendocrine cells is successfully demonstrated.
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影响因子：
4.6
作者：
Zheng, Yi;He, Zhizhu;Gao, Yunxia;Liu, Jing
通讯作者：
Liu, Jing
DOI：
10.1007/s11663-009-9276-5
发表时间：
2009-08
期刊：
Metallurgical and Materials Transactions B
影响因子：
--
作者：
B. Zheng;Yaojun Lin;Yizhang Zhou;E. Lavernia
通讯作者：
B. Zheng;Yaojun Lin;Yizhang Zhou;E. Lavernia
影响因子：
1.6
作者：
Morley, N. B.;Burris, J.;Nornberg, M. D.
通讯作者：
Nornberg, M. D.
影响因子：
6.7
作者：
Benguettat O;Jneid R;Soltys J;Loudhaief R;Brun-Barale A;Osman D;Gallet A
通讯作者：
Gallet A
影响因子：
3.4
作者：
Neumann TV;Kara B;Sargolzaeiaval Y;Im S;Ma J;Yang J;Ozturk MC;Dickey MD
通讯作者：
Dickey MD