Metabolic interactions between dynamic bacterial subpopulations
Metabolic interactions between dynamic bacterial subpopulations
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DOI：
10.7554/elife.33099
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发表时间：
2018-05-29
期刊：
影响因子：
7.7
通讯作者：
Elowitz, Michael B.
中科院分区：
文献类型：
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作者：
Rosenthal, Adam Z.;Qi, Yutao;Elowitz, Michael B.
已知单个微生物物种在多物种群落中占据独特的代谢生态位。然而，目前尚不清楚克隆细菌群体中是否可以类似地发生代谢专门化。更具体地说，尚不清楚这种专业化可以提供哪些功能以及如何动态协调专业化。在这里，我们表明，呈指数增长的枯草芽孢杆菌培养物分为不同的相互作用的代谢亚群，包括产生乙酸的一个群体，以及差异表达用于产生乙偶姻（一种pH中性储存分子）的代谢基因的另一个群体。这些亚群表现出不同的增长率和状态之间的动态相互转换。此外，乙酸盐浓度影响不同亚群的相对大小。这些结果表明，克隆群体可以利用代谢专门化，通过动态的、环境敏感的状态转换过程来控制环境。
Individual microbial species are known to occupy distinct metabolic niches within multi-species communities. However, it has remained largely unclear whether metabolic specialization can similarly occur within a clonal bacterial population. More specifically, it is not clear what functions such specialization could provide and how specialization could be coordinated dynamically. Here, we show that exponentially growing Bacillus subtilis cultures divide into distinct interacting metabolic subpopulations, including one population that produces acetate, and another population that differentially expresses metabolic genes for the production of acetoin, a pH-neutral storage molecule. These subpopulations exhibit distinct growth rates and dynamic interconversion between states. Furthermore, acetate concentration influences the relative sizes of the different subpopulations. These results show that clonal populations can use metabolic specialization to control the environment through a process of dynamic, environmentally-sensitive state-switching.