PHYSICAL DELINEATION OF THE MINIMAL CHROMOSOMAL SEGMENT ENCOMPASSING THE MURINE HOST-RESISTANCE LOCUS BCG
PHYSICAL DELINEATION OF THE MINIMAL CHROMOSOMAL SEGMENT ENCOMPASSING THE MURINE HOST-RESISTANCE LOCUS BCG
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DOI：
10.1006/geno.1993.1388
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发表时间：
1993-09-01
期刊：
影响因子：
4.4
通讯作者：
GROS, P
中科院分区：
文献类型：
--
作者：
MALO, D;VIDAL, S;GROS, P
宿主抗性基因座Bcg决定小鼠对细胞内病原体感染的抗性，所述细胞内病原体例如某些种类的分枝杆菌、鼠伤寒沙门氏菌和杜氏利什曼原虫。Bcg定位于小鼠1号染色体的近端部分，与绒毛蛋白（Vil）紧密连锁，基因排列顺序为Tp-1-（1 cM）-D1 Mcg 105-（0.1 cM）-λ Mm 1C 165/Vil/Bcg-（0.2 cM）-λ Mm 1C 136-（0.3 cM）-Des-（0.1 cM）-Inha。为了克隆Bcg的基因组序列，我们使用脉冲场凝胶电泳（PFGE）和荧光原位杂交构建了近端小鼠1号染色体的3.9-Mb片段的物理图谱，nearBcg.In原位杂交中期小鼠染色体表明，映射的区域是带C5内。TP-1-Vil和λ Mm 1C 136-Inha间隔的物理定位用PFGE分析，Vil-Desha间隔用间期核原位杂交估算。这些组合分析的结果显示了如下的位点顺序和最大位点间距离：Tp-1-（1000 kb）-D1 Mcg 105-（160 kb）-λ Mm 1C 165-（180 kb）-Vil-（800 kb）-λ Mm 1C 136-（290 kb）-Des-（130 kb）-Inha。该区域的详细限制性酶切图谱鉴定了许多假定的CpG岛，表明Bcg附近存在几个转录单位。
The host resistance locusBcgdetermines resistance of mice to infection with intracellular pathogens such as certain species ofMycobacteria, Salmonella typhimurium, andLeishmania donovani.Bcgmaps on the proximal portion of mouse chromosome 1, very tightly linked to villin (Vil), with the gene order and intergene distancesTp-1-(1 cM)-D1Mcg105-(0.1 cM)-λMm1C165/Vil/Bcg-(0.2 cM)-λMm1C136-(0.3 cM)-Des-(0.1 cM)-Inha. In an effort to clone genomic sequences overlappingBcg, we have used pulsed-field gel electrophoresis (PFGE) and fluorescencein situhybridization to construct a physical map of the 3.9-Mb segment of proximal mouse chromosome 1, nearBcg.In situhybridization to metaphase mouse chromosomes indicates that the mapped region is within band C5. Physical mapping of theTp-1-Viland λMm1C136-Inhaintervals was carried out by PFGE analysis, whereas theVil-Desinterval was estimated byin situhybridization to interphase nuclei. Results of these combined analyses indicate the following locus order and maximal interlocus distances:Tp-1-(1000 kb)-D1Mcg105-(160 kb)-λMm1C165-(180 kb)-Vil-(800 kb)-λMm1C136-(290 kb)-Des-(130 kb)-Inha. Detailed restriction mapping of this region identifies numerous putative CpG islands, suggesting that several transcription units are present in the vicinity ofBcg.