Growth dynamics of Artemisia annua hairy roots in three culture systems
Growth dynamics of Artemisia annua hairy roots in three culture systems
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DOI：
10.1002/bit.10685
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发表时间：
2003-04-20
影响因子：
3.8
通讯作者：
Wyslouzil, BE
中科院分区：
文献类型：
--
作者：
Kim, YJ;Weathers, PJ;Wyslouzil, BE
将青蒿毛状根的瞬时生长与在摇瓶、鼓泡塔和雾化反应器中生长的培养物进行比较。通过对瞬时生物量测量值进行数值微分来获得瞬时增长率。具体糖消耗率与文献值非常吻合。根据生长速率和糖消耗速率，确定了所有三种培养系统的比产量和糖维持系数。对于在摇瓶和泡罩柱中生长的根来说，这些值在统计上无法区分。相比之下，在泡罩塔和雾化反应器中生长的根的值在统计上是不同的，这表明在这两个系统中生长的根的糖利用可能不同。通过测量鼓泡塔反应器中的呼吸速率，我们还确定了实际的生物量产量以及 O-2 和 CO2 的维持系数，再加上对根部的元素分析，这使我们能够获得合理的碳平衡。 (C) 2003 年 Wiley 期刊公司。
The transient growth of Artemisia annua hairy roots was compared for cultures grown in shake flasks and in bubble column and mist reactors. Instantaneous growth rates were obtained by numerically differentiating the transient biomass measurements. Specific sugar consumption rates showed good agreement with literature values. From the growth rate and sugar consumption rate, the specific yield and maintenance coefficient for sugar were determined for all three culture systems. These values were statistically indistinguishable for roots grown in shake flasks and bubble columns. In contrast, the values for roots grown in bubble columns and mist reactors were statistically different, suggesting that sugar utilization by roots grown in these two systems may be different. By measuring respiration rates in the bubble column reactor we also determined the actual biomass yield and maintenance coefficient for O-2 and CO2 Together with an elemental analysis of the roots, this allowed us to obtain a reasonable carbon balance. (C) 2003 Wiley Periodicals, Inc.